The effects of antibiotics and surfactant addition on methionine accumulation in the broth culture of three strains of methionine yeilding Bacillus cereus (B. cereus DS-13, B. cereus AS-9 and B. cereus RS-16) recovered from different soil ecovars in Owerri Nigeria were investigated. Ampicillin and erythromycin neither stimulated growth nor enhanced methionine production in Bacillus cereus DS-13, while tetracycline and chloramphenicol stimulated growth and enhanced methionine yield in Bacillus cereus DS-13 and Bacillus cereus AS-9. Ampicllin had the highest stimulatory effect on growth and methionine production in Bacillus cereus RS 16. Tween 80 and palmitic acid stimulated growth and improved methionine accumulation in the broth culture of all Bacillus strains while stearic acid did not improve yield in all Bacillus strains.