Background:
Chemokines are small proteins that activate immune system in normal and
pathological conditions. The induction of chemotaxis is a well-established
role of chemokines. Moreover chemokines are important mediators of
angiogenesis, implantation of fetus, and maturation of immune cells. In human
body many types of cells express chemokines and cytokines at level of gene and
protein. In blood cells chemokine and chemokine receptors mRNA level is a one
of crucial points of chemokine system condition. The aim of the study was to
evaluate the relationship between plasma concentration of cyto- kines and
chemokines/chemokine receptors mRNA level in blood of healthy volunteers. Results:
Gene expression of eotaxin, eotaxin-2, IL-8, MIP-1α, MIP- 1β, RANTES, CCR1,
CCR3, CCR5, CXCR1, and CXCR2 was measured in peripheral blood cells, as well as
the concentration of IL-1β, IL-1ra,
IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p70), IL-13, IL-15,
IL-17, eotaxin, FGF-2, G-CSF, GM-CSF, IFN-γ,
IP-10, MCP-1, MIP-1α, MIP-1β, PDGF-BB, RANTES, TNF-α, and VEGF was evaluated in the plasma
of 19 healthy individuals. We studied rela- tionship between mRNA levels of
chemokines/recaptors and cytokine concentration in blood of healthy
volunteers. Conclusion: These data are allowed to assess chemokines impact in
the cytokine regulation of healthy subjects. These results indicate that chemokines
and their receptors is diverse and redundant system of immune reactivity in response
to internal and external challenges.