The extracted fish samples (w/w) using
pressurized liquid extraction (PLE) were cleaned up by applying different adsorbent
materials such as: combination of acidic/ basic silica and alumina, florisil,
and silica gel either alone or in combination with another sort of sorbent.
Different solvents such as hexane (H), dichloromethane (DCM), with different
compositions were used to elute the PCBs target compounds from the spiked fish
samples. Overall the mean percentage recoveries for all PCBs congeners using
PLE were ranging from 78.6% to 98.7%; from 79.4% to 91.8%; from 65.8% to 104.5%
and from 82.4% to 100.9% for cases A, B, C, D and E, respectively. However, the
overall mean percentage recoveries for the 13C-PCBs surrogate including: 101, 138, 153, 180 and
209 were ranging from 88.2% to 97.6%; from 79.4% to 90.2%, from 88.3% to 96.4%,
from 79.8% to 90.4% and from 83.9% to 95.9%, for cases A, B, C, D and E,
respectively. The limits of detection of the proposed method were varied from
0.01 to 0.28 ng/g (w/w) for different PCBs congeners. The proposed method has
linear dynamic range from 21.5 ng/g to 109 ng/g. This method was applied to fish samples for the
determination of the target PCBs components.