Author(s)

Khagga Bhavyasri, D. Rambabu, P. S. S. Prasad, V. Murali Balaram

Agilent Technologies India Pvt. Ltd., Hyderabad, India.
Center for Pharmaceutical Sciences Department, Jawaharlal Nehru Technological University, Hyderabad, India.
Drug Control, Hyderabad, India.
Sultan Ul-Uloom College of Pharmacy, Hyderabad, India.

A packed column supercritical fluid chromatography (SFC) method for the separation of clopidogrel enantiomers on a chiral stationary phase and CO₂ with modifier as mobile phase has been developed at an analytical scale. Among 11 different 2 stationary phases the Chiral cel OD-H column showed by far the best separation properties. The influence of different modifiers, injection solvents, temperature, and pressure, and density of the fluid, respectively, on the separation behaviour has been studied. It was found that the separation behaviour strongly depends on the type of modifier and the modifier content. Temperature and pressure are of less influence.

Chiral Stationary Phases; SFC; Enantiomer Separation; Supercritical Fluid Chromatography; Clopidogrel

K. Bhavyasri, D. Rambabu, P. Prasad and V. Balaram, "Separation of Enantiomers of Clopidogrel on Chiral Stationary Phases by Packed Column Supercritical Fluid Chromatography," American Journal of Analytical Chemistry, Vol. 4 No. 1, 2013, pp. 51-55. doi: 10.4236/ajac.2013.41007.