Author(s)

Dai Long Vu, Libor Červenka, Jana Vavřičková

Department of Analytical Chemistry, University of Pardubice, Pardubice, Czech Republic.

The attachment of Staphylococcus epidermidis to the surface of carbon paste electrode (CPE) by applying positive potentials (50 - 600 mV) with regard of various buffer pH, cultivation time and solid-medium type was studied. The attachment process was analyzed by measuring the electric current derived from the dye (amido black) adsorbed on the vacant areas of CPE after attachment of microbial cells. The pH was not identified as a single main factor affecting the attachment (p > 0.05), however further insight revealed that the potentials applied had different effects on the microbial cells attachment. Both cultivation time and solid-medium type significantly affected the microbial attachment. Generally, increase of cultivation time up to 168 h resulted in increase of adhesion. Applying potentials 300, 400 and 200 mV resulted in the highest attachment process for bacteria cultivated for 24, 48 and 168 h, respectively. S. epidermidis cultivated on the blood agar and Baird-Parker agar plates showed the higher extent of attachment.

Carbon Paste Electrode; Attachment; Staphylococcus epidermidis

Vu, D. , Červenka, L. and Vavřičková, J. (2012) The attachment of Staphylococcus epidermidis on the surface of a carbon paste electrode at various positive potentials: The effect of pH, incubation time, and solid-medium type. Journal of Biomedical Science and Engineering, 5, 699-704. doi: 10.4236/jbise.2012.512087.