Author(s)

Javier García-Ferreyra, Pedro Valdivieso, Milton Zambrano, Jorge Carpio

FERTILAB Laboratory of Assisted Reproduction, Lima, Perú.
Laboratory of Assisted Reproduction, Alcival Hospital, Guayaquil, Ecuador.

Purpose: Evaluate the effect of preincubation of oocytes prior to IVF or ICSI cycles with embryo transfer at blastocyst stage. Methods: Retrospective non randomized study based on secondary analysis of data. Setting: Laboratory of Assisted Reproduction at the Alcivar Hospital. Patients: One hundred-eighteen cycles of IVF and ICSI were analyzed in the present study. The evaluated groups were formed for those patients whose oocytes, after retrieval, were inseminated at 1-3 h (Group I) or 4-6 h (Group II). Results: There was no difference in fertilization rate (83.6% and 78.1%), Day 3 cleavage rate (95.1% and 97.1%), and blastocyst formation (31.1% and 39.1%) for groups I and II respectively. Clinical pregnancy rates (PR: 53.0% vs 22.9%) and implantation rates (IR: 38.1% vs 13.0%) were significantly higher in group II versus group I, respectively (P < 0.05). Conclusions: Preincubation of oocytes before insemination is a factor which raises the PR and IR after the blastocyst transfer.

Oocytes; Embryo Development; Maturation; Blastocyst; ART

García-Ferreyra, J. , Valdivieso, P. , Zambrano, M. and Carpio, J. (2012) High pregnancy and implantation rates can be obtained with preincubation of oocytes before insemination in IVF and ICSI procedures. Health, 4, 660-666. doi: 10.4236/health.2012.49104.