Author(s)

Laura Luzuriaga, María Fernanda Cerdá

Laboratorio de Biomateriales, Facultad de Ciencias, Montevideo, Uruguay.

The interaction of compounds with potential use as pharmaceutical with a carrier protein as serum albumin is of great importance in their biodistribution. Albumin offers different sites for binding metallic compounds. Using a combination of spectropho-tometric and electrochemical techniques, the interaction between [Ru(phen)₃]Cl₂ (phen = phenantroline) and bovine serum albumin was evaluated. In particular, it was possible to calculate an apparent binding constant (Kb) of 4.4 × 103 (for concentrations expressed in M) for the main interaction site of the protein. A number of ca. 40 molecules of Ru-phen per molecule of BSA under saturation conditions, and a positive cooperative behavior towards association from the protein were found.

Saturation Graph; Spectrophotometry; Cyclic Voltammetry

Luzuriaga, L. and Cerdá, M. (2012) Analysis of the interaction between [Ru(phenanthroline)₃]²⁺ and bovine serum albumin. Advances in Biological Chemistry, 2, 262-267. doi: 10.4236/abc.2012.23033.