Author(s)

Sabah Boumerfeg, Abderrahmane Baghiani, Meriem Djarmouni, Djamila Ameni, Moufida Adjadj, Farida Belkhiri, Noureddine Charef, Seddik Khennouf, Lekhmici Arrar

Department of Biology, University Bachir El-Ibrahimi, Bordj Bou Arreridj, Algeria.
Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif, Algeria.
Laboratory of Chronic Diseases, Department of Biology and Physiology, Faculty of Nature and Life Sciences, University Ferhat Abbas, Setif, Algeria.

The antioxidative activities of subfractions; methanol (CE), chloroform (CHE) and ethyl acetate (EAE) of Teucrium polium extracts (TPE) were investigated. HPLC analysis of the plant revealed the existence of procyanidins B₁ and B₂, gallic acid, catechin and epicatechin. All the extracts showed inhibitory properties on xanthine oxidase, with IC₅₀ ranging from 0.80 ± 0.07 to 11.76 ± 0.50 μM/quercetin equivalent. In the cellular system, all the extracts showed a protec-tive effect greater than those of quercetin, rutin and gallic acid against t-BHP induced oxidative damages in human erythrocytes. These results were clearly confirmed by a modified thiobarbituric acid-reactive species (TBARS), and β-carotene/linoleic acid assay which demonstrated that CHE possess an inhibition ratio of the linoleic acid oxidation (83.11%) close to that of BHT (96.77%). In addition, the results showed that the extracts possess a potent DPPH radical scavenging activity and gave a reduction power greater than rutin, quercetin, gallic acid and ascorbic acid in FRAP assay. These results show that Teucrium polium extracts have strong antioxidant effects and may have some clinical benefits.

Xanthine Oxidase; Antioxidant; Superoxide Scavenger; Teucrium polium

Boumerfeg, S. , Baghiani, A. , Djarmouni, M. , Ameni, D. , Adjadj, M. , Belkhiri, F. , Charef, N. , Khennouf, S. and Arrar, L. (2012) Inhibitory Activity on Xanthine Oxidase and Antioxidant Properties of Teucrium polium. Chinese Medicine, 3, 30-41. doi: 10.4236/cm.2012.31006.