Prof. Gary
W. Black
Department of Applied Sciences, Faculty of Health and Life Sciences
Northumbria University, UK
Email: gary.black@northumbria.ac.uk
Qualifications
1992 PhD, Biochemistry and Molecular Biology. Awarded by the Council for
National Academic Awards, UK.
1988 BSc, Applied Biology. Awarded by the Council for National Academic
Awards, UK.
Publications (Selected)
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Malik V, Zhang M, Dover LG, Northen JS, Flinn A,
Perry JJ, Black GW. (2013) Sterol 3β-glucosyltransferase biocatalysts with a
range of selectivities, including selectivity for testosterone. Molecular
BioSystems 9, 2816-2822.
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Karabencheva T, Carlsson U, Balali-Mood K, Black
GW, Christov CZ. (2013) Conformational Effects on the Circular Dichroism of
Human Carbonic Anhydrase II: A Multilevel Computational Study PLoS ONE 8(2): e56874.
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Malik V, Black GW. (2012) Structural, functional
and mutagenesis studies of UDP-glycosyltransferases. Advances in Protein
Chemistry and Structural Biology 87,
87-115.
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Yang Q, Porter AJ, Zhang M, Harrington DJ, Black
GW, Sutcliffe IC. (2012) The impact of pH and nutrient stress on the growth and
survival of Streptococcus agalactiae. Antonie van Leeuwenhoek
International Journal of General and Molecular Microbiology 102, 277–287.
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Yang Q, Zhang M, Harrington DJ, Black GW,
Sutcliffe IC. (2011) A proteomic investigation of Streptococcus agalactiae
reveals that human serum induces the C protein β antigen and arginine
deiminase. Microbes and Infection 13, 757-760.
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van Pelt S,
Zhang M, Otten LG, Holt J, Sorokin DY, van
Rantwijk F, Black GW, Perry JJ, Sheldon RA. (2011) Probing the
Enantioselectivity of a Diverse Group of Purified Cobalt-Centred Nitrile
Hydratases. Organic & Biomolecular Chemistry 9, 3011-9.
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Zainab H. Elmabrouk ZH, Vincent
F, Zhang M, Smith NL, Turkenburg JP,
Charnock SJ, Black GW, Taylor EJ. (2011) Crystal structures of a family 8 polysaccharide lyase reveal
open and highly occluded substrate-binding cleft conformations. Proteins:
Function, Structure and Bioiformatics 79, 965–974.
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Yang Q, Zhang M, Harrington DJ, Black GW,
Sutcliffe IC. (2010) A proteomic investigation of Streptococcus agalactiae
grown under conditions associated with neonatal exposure reveals the
upregulation of the putative virulence factor C protein beta antigen. International
Journal of Medical Microbiology 300, 331–337.
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Black GW, Gregson T, McPake CB, Perry JJ,
Zhang M. (2010) Biotransformation of nitriles using the solvent-tolerant
nitrile hydratase fromRhodopseudomonas palustris CGA009. Tetrahedron
Letters 51, 1639-1641.
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Martinez-Fleites C, Smith NL, Turkenburg JP,
Black GW, Taylor EJ. (2009) Structures of two truncated phage-tail hyaluronate
lyases fromStreptococcus pyogenes serotype M1. Acta
Crystallographica Section F-Structural Biology and crystallization
Communications 65, 963-966.
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Lindsay A-M, Zhang M, Mitchell Z, Holden MTG,
Waller AS, Sutcliffe IC, Black GW. (2009) The Streptococcus equi
prophage-encoded protein SEQ2045 is a hyaluronan-specific hyaluronate lyase
that is produced during equine infection. Microbiology-SGM 155,
443-449.
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Sutcliffe IC, Black GW, Harrington DJ. (2008)
Bioinformatics insights into the biosynthesis of the Group B carbohydrate in
Streptococcus agalactae.Microbiology-SGM 154,
1354-1363.
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Zhang M, McDonald FM, Sturrock SS, Charnock
SJ, Humphery-Smith I, Black GW. (2007) Group A streptococcus cell-associated
pathogenic proteins as revealed by growth in hyaluronic acid-enriched
media. Proteomics 7, 1379-1390.
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Sheldon WL, Macauley MS, Taylor EJ, Charnock
SJ, Davies GJ, Vocadlo DJ, Black GW. (2006) Functional analysis of a group A
streptococcal glycoside hydrolase from family 84 reveals it is a
beta-N-acetylglucosaminidase and not a hyaluronidase. Biochemical
Journal 399, 241-247
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Dennis RJ, Taylor EJ, Macauley MS, Stubbs KA,
Turkenburg JP, Hart SJ, Black GW, Vocadlo DJ, Davies GJ. (2006) Structure and
mechanism of a bacterial beta-glucosaminidase having O-GlcNAcase
activity. Nature Structural & Molecular Biology 13,
365-371.
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Smith NL, Taylor EJ, Lindsay A-M, Charnock
SJ, Turkenburg JP, Dodson EJ, Davies GJ, Black GW. (2005) Structure of a group
A streptococcal phage-encoded virulence factor reveals a catalytically-active
triple-stranded β-helix. Proceedings of the National Academy of
Sciences (USA) 102, 17652-17657.
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O'Mahoney
R, Doran J, Coffey L, Cahill OJ, Black GW, O'Reilly C. (2005) Characterisation
of the nitrile hydratase gene clusters of Rhodococcus erythropolis strains
AJ270 and AJ300 and Microbacterium sp. AJ115 indicates
horizontal gene transfer and reveals an insertion of IS1166.Antonie van
Leeuwenhoek International Journal of General and Molecular Microbiology.
87, 221-32.
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Taylor
EJ, Smith NL, Colby J, Charnock SJ, Black GW (2004). The gene encoding the
ribulose monophosphate pathway enzyme, 3-hexulose-6-phosphate synthase, from
Aminomonas aminovorus C2A1 is adjacent to coding sequences that exhibit
similarity to histidine biosynthesis enzymes.Antonie van Leeuwenhoek
International Journal of General and Molecular Microbiology 86,
167-172.
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Humphry
DR, Black GW, Cummings SP. (2003) Reclassification of'Pseudomonas
fluorescens subsp. cellulosa' NCIMB 10462 (Ueda et
al. 1952) as Cellvibrio japonicus sp. nov. and
revival of Cellvibrio vulgaris sp. nov., nom. rev. and Cellvibrio
fulvus sp. nov., nom. rev. International Journal of Systematic
and Evolutionary Microbiology 53, 393-400.
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Charnock
SJ, Brown IE, Turkenburg JP, Black GW, Davies GJ. (2002) Convergent evolution
sheds light on the anti-b-elimination mechanism common to family 1
and 10 polysaccharide lyases. Proceedings of the National Academy of
Sciences (USA) 99, 12067-12072.
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Czjzek M,
Bolam DN, Mosbah A, Allouch J, Fontes CMGA, Ferreira LMA, Bornet O, Zamboni V,
Darbon H, Smith NL, Black GW, Henrissat B, Gilbert HJ. (2001) The location of the
ligand binding site of carbohydrate binding modules that have evolved from a
common sequence is not conserved.Journal of Biological Chemistry 276,
48580-48587.
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Taylor
EJ, Charnock SJ, Colby J, Davies GJ, Black GW. (2001) Cloning, purification and
characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus
subtilis. Acta Crystallographica D57, 1138-1140.
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Charnock
SJ, Brown IE, Turkenburg JP, Black GW, Davies GJ. (2001) Characterization of a
novel pectate lyase, Pel10A, from Pseudomonas cellulosa. Acta
Crystallographica D57, 1141-1143.
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Humphry
DR, George A, Black GW, Cummings SP. (2001) Flavobacterium frigidarium sp
nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from
Antarctica. International Journal of Systematic and Evolutionary
Microbiology 51, 1235-1243.
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Brown IE,
Mallen MH, Charnock SJ, Davies GJ, Black GW. (2001) Pectate lyase 10A
from Pseudomonas cellulosa is a modular enzyme containing a
family 2a carbohydrate-binding module. Biochemical Journal 355,
155-165.
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Colby J,
Snell D, Black GW. (2000) Immobilization of Rhodococcus AJ270
and use of entrapped biocatalyst for the production of acrylic acid.Chemistry
Monthly 131, 655-666.
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Cummings
SP, Black GW. (1999) Polymer hydrolysis in a cold climate.Extremophiles 3,
81-87.
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Braithwaite
KL, Barna T, Spurway TD, Charnock SJ, Black GW, Hughes N, Lakey JH, Virden R,
Hazlewood GP, Henrissat B, Gilbert HJ. (1997) Evidence that galactanase A
from Pseudomonas fluorescens subspecies cellulosa is
a retaining family 53 glycosyl hydrolase in which E161 and E270 are the
catalytic residues. Biochemistry 36, 15489-15500.
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Black GW,
Rixon JE, Clarke JH, Hazlewood GP, Ferreira LMA, Bolam DN, Gilbert HJ. (1997)
Cellulose binding domains and linker sequences potentiate the activity of hemicellulases
against complex substrates. Journal of Biotechnology 57,
59-69.
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McKie VA,
Black GW, MillwardSadler SJ, Hazlewood GP, Laurie JI, Gilbert HJ. (1997)
Arabinanase A from Pseudomonas fluorescens subsp cellulosa exhibits
both an endo- and an exo- mode of action. Biochemical Journal 323,
547-555.
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Black GW,
Rixon JE, Clarke JH, Hazlewood GP, Theodorou MK, Morris P, Gilbert HJ. (1996)
Evidence that linker sequences and cellulose-binding domains enhance the
activity of hemicellulases against complex substrates.Biochemical Journal 319,
515-520.
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Millward-Sadler
SJ, Hall J, Black GW, Hazlewood GP, Gilbert HJ. (1996) Evidence that the
Piromyces gene family encoding endo-1,4-mannanases arose through gene
duplication. FEMS Microbiology Letters 141, 183-188.
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Fanutti
C, Ponyi T, Black GW, Hazlewood GP, Gilbert HJ. (1995) The conserved
noncatalytic 40-residue sequence in cellulases and hemicellulases from
anaerobic fungi functions as a protein docking domain. Journal of
Biological Chemistry 270, 29314-29322.
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Millward-Sadler
SJ, Davidson K, Hazlewood GP, Black GW, Gilbert HJ, Clarke JH. (1995) Novel
cellulose-binding domains, nodB homologs and conserved modular architecture in
xylanases from the aerobic soil bacteriaPseudomonas fluorescens subsp cellulosa and cellvibrio
mixtus. Biochemical Journal 312, 39-48.
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Hall J,
Black GW, Ferreira LMA, Millward-Sadler SJ, Ali BRS, Hazlewood GP, Gilbert HJ.
(1995) The noncatalytic cellulose-binding domain of a novel cellulase
from Pseudomonas fluorescens subsp. cellulosa is
important for the efficient hydrolysis of avicel. Biochemical Journal 309,
749-756.
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Black GW,
Hazlewood GP, Millward-Sadler SJ, Laurie JI, Gilbert HJ. (1995) A modular
xylanase containing a novel noncatalytic xylan-specific binding domain. Biochemical
Journal 307, 191-195
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Braithwaite
KL, Black GW, Hazlewood GP, Ali BRS, Gilbert HJ. (1995) A non-modular
endo-b-1,4-mannanase from Pseudomonas fluorescens subspeciescellulosa. Biochemical
Journal 305, 1005-1010.
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Ali BRS,
Zhou LQ, Graves FM, Freedman RB, Black GW, Gilbert HJ, Hazlewood GP. (1995)
Cellulases and hemicellulases of the anaerobic fungusPiromyces constitute
a multiprotein cellulose-binding complex and are encoded by multigene
families. FEMS Microbiology Letters 125, 15-21.
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Pearson DM,
O’Reilly C, Colby J, Black GW. (1994) DNA sequence of the cut a, cut
b and cut c genes, encoding the molybdenum-containing
hydroxylase carbon-monoxide dehydrogenase, from Pseudomonas
thermocarboxydovorans strain C2. Biochimica et Biophysica Acta 1188,
432-438.
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Black GW,
Hazlewood GP, Xue GP, Orpin CG, Gilbert HJ. (1994) Xylanase B from Neocallimastix
patriciarum contains a noncatalytic 455-residue linker sequence
comprised of 57 repeats of an octapeptide. Biochemical Journal299,
381-387.
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Zhou LQ, Xue
GP, Orpin CG, Black GW, Gilbert HJ, Hazlewood GP (1994) Intronless celB from
the anaerobic fungus Neocallimastix patriciarumencodes a modular
family a-endoglucanase. Biochemical Journal 297, 359-364.
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Ferreira
LMA, Wood TM, Williamson G, Faulds C, Hazlewood GP, Black GW, Gilbert HJ.
(1993) A modular esterase from Pseudomonas fluorescens subspcellulosa contains
a noncatalytic cellulose-binding domain. Biochemical Journal 294,
349-355.
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O’Reilly
C, Black GW, Laffey R, McConnell DJ. (1990) Molecular analysis of an IS200
insertion in the gpt gene of Salmonella typhimurium LT2. Journal
of Bacteriology 172, 6599-6601.
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Barstow
DA, Black GW, Sharman AF, Scawen MD, Atkinson T, Li SS, Chia WN, Clarke AR,
Holbrook JJ. (1990) Expression of the copy DNA for humana4 and b4
L-lactate dehydrogenases in Escherichia coli. Biochimica et Biophysica
Acta 1087, 73-79.
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Black GW,
Lyons CM, Williams E, Colby J, Kehoe M, O’Reilly C. (1990) Cloning and
expression of the carbon-monoxide dehydrogenase genes fromPseudomonas
thermocarboxydovorans strain C2. FEMS Microbiology Letters70, 249-254.