Prof. Arun K. Bhunia

Department of Food Science

Department of Comparative Veterinary Pathobiology

Purdue Institute of Inflammation, Immunology, and Infectious Diseases

Purdue University

West Lafayette, IN, USA

Email: bhunia@purdue.edu

Qualifications

1995 Postdoctoral Training, University of Arkansas, Fayetteville, AR, USA

1989 Ph.D., University of Wyoming, Laramie, USA

1984 Bachelor of Veterinary Science (equivalent to DVM), WBUAFS, India

Selected Publications

1. Bai, X., Liu, D., Xu, L., Drolia, R., Gallina, L.F., Cox, A.D., and Bhunia, A.K. 2021. Biofilm-isolated Listeria monocytogenes exhibits reduced systemic dissemination at the early (12-24 h) stage of infection in a mouse model. Npj Biofilm and Microbiome 7:18
2. Ryan, V.E., Taylor W. Bailey, Liu, D., T.W., Vemulapalli, T., Cooper, B., Durkes, A.C., and Bhunia, A.K. 2021. Listeria adhesion protein-expressing bioengineered probiotics prevent fetoplacental transmission of Listeria monocytogenes in a pregnant guinea pig model. Microbial Pathogenesis 151:104752
3. Drolia, R., Amalaradjou, M.A.R., Ryan, V.E., Tenguria, S., Liu, D., Bai., X., Xu, L., Singh, A.K., Cox, A.D., Bernal-Crespo, V., Schaber, J.A., Applegate, B.M., Vemulapalli, R., and Bhunia, A.K. 2020. Receptor-targeted engineered probiotics mitigate lethal Listeria infection. Nature Communications 11:6344.
4. Xu, L., Bai, X., Tenguria, S., Liu, Y., Drolia, R., and Bhunia, A.K. 2020. Mammalian cell-based immunoassay for detection of viable bacterial pathogens. Frontiers in Microbiology 11:575615
5. Drolia, R., and Bhunia, A.K. 2019. Crossing the Epithelial Barrier via Listeria Adhesion Protein and Internalin A, Trends in Microbiology 27 (5): 408-425.
6. Mathipa, M., Thantsha, M.S., and Bhunia, A.K., 2019. Lactobacillus casei expressing internalins A and B reduces Listeria monocytogenes interaction with Caco-2 cells in vitro. Microbial Biotechnology 12(4):715-729.
7. Drolia, R., Tenguria, S., Durkes, A.C., Turner, J.R. and Bhunia, A.K. 2018. Listeria Adhesion Protein Induces Intestinal Epithelial Barrier Dysfunction for Bacterial Translocation. Cell Host & Microbe 23(4): 470-480.e7.
8. Zhu, X., Liu, D., Singh, A.K., Drolia, R., Bai, X., Tenguria, S., and Bhunia, A.K. 2018. Tunicamycin mediated inhibition of wall teichoic acid affect Staphylococcus aureus and Listeria monocytogenes cell morphology, biofilm formation and virulence. Frontiers in Microbiology 9:1352.
9. Koo, O.K., Amalaradjou, M.A.R., and Bhunia, A.K. 2012. Recombinant probiotic expressing Listeria adhesion protein attenuates Listeria monocytogenes virulence in vitro. PLoS One 7 (1):e29277.
10. Jagadeesan, B., Fleishman Littlejohn, A.E., Amalaradjou, M.A.R., Singh, A.K., Mishra, K., La, D., Kihara, D., and Bhunia, A.K. 2011. N-terminal Gly224–Gly411 domain in Listeria Adhesion Protein interacts with host receptor Hsp60. PLoS ONE 6 (6):e20694.
11. Burkholder, K.M. and Bhunia, A.K. 2010. Listeria monocytogenes uses LAP to promote bacterial transepithelial translocation, and induces expression of LAP receptor Hsp60. Infection and Immunity 78(12):5062-5073.
12. Jagadeesan, B., Koo, O.K., Kim, K.-P., Burkholder, K.M., Mishra, K.K., Aroonnual, A., and Bhunia, A.K. 2010. LAP, an alcohol acetaldehyde dehydrogenase enzyme in Listeria promotes bacterial adhesion to enterocyte-like Caco-2 cells only in pathogenic species. Microbiology-UK. 156 (9):2782–2795.
13. Burkholder, K.M., Kim, K-P., Mishra, K., Medina, S., Hahm, B-K., Kim, H., and Bhunia, A.K. 2009. Expression of LAP, a SecA2-dependent secretory protein, is induced under anaerobic environment. Microbes and Infection 11:859-867.
14. Koo, O.K., Liu, Y., Shuaib, S., Bhattacharya, S., Ladisch, M.R., Bashir, R., and Bhunia, A.K. 2009. Targeted capture of pathogenic bacteria using mammalian cell receptor coupled with dielectrophoresis on biochip. Analytical Chemistry 81:3094-3101. 15. Banerjee, P., Lenz, D., Robinson, J.P, Rickus, J.L., and Bhunia, A.K. 2008. A novel and simple cell-based detection system with collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins. Laboratory Investigation 88:196-206.
15. Akin, D., Sturgis, J., Ragheb, K., Sherman, D., Burkholder, K., Robinson, J.P., Bhunia, A.K., Mohammed, S. and Bashir, R. 2007. Bacteria-mediated delivery of nanoparticles into cells. Nature Nanotechnology 2:441-449.
16. Kim, K.P., Jagadeesan, B., Burkholder, K.M., Jaradat, Z.W., Wampler, J.L., Lathrop, A.A., Morgan, M.T. and Bhunia, A.K. 2006. Adhesion characteristics of Listeria adhesion protein (LAP) – expressing Escherichia coli to Caco-2 cells and of recombinant LAP to eukaryotic receptor Hsp60 as examined in a surface plasmon resonance sensor. FEMS Microbiology Letters 256:324-332.
17. Gray, K.M., Banada, P.P., O'Neal, E. and Bhunia, A.K. 2005. Rapid Ped-2E9 cell-based cytotoxicity analysis and genotyping of Bacillus species. Journal of Clinical Microbiology 43 (12):5865-5872.
18. Maldonado, Y., Fiser, J.C., Nakatsu, C.H. and Bhunia, A.K. 2005. Cytotoxicity potential and genotypic characterization of Escherichia coli isolates from environmental and food sources. Applied and Environmental Microbiology 71 (4):1890-1898.
19. Wampler, J.L., Kim, K.P., Jaradat, Z. and Bhunia, A.K. 2004. Heat-shock protein 60 acts as a receptor for the Listeria adhesion protein in Caco-2 cells. Infection and Immunity 72(2):931-936.

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