Dr. Clark Ford

Iowa State University, USA

Email:cfford@iastate.edu

Qualifications

1992  Associate Professor of Food Science and Human Nutrition
1985  Assistant Professor, Iowa State University, Genetics
1982  Postdoctor, University of Virginia, Molecular Biology
1981  Ph.D., University of Iowa, Genetics

Published(Selected)

1. Ford, C., S. Mitchell, and W.‑Y. Wang. (1981). Protochlorophyllide photoconversion mutants of Chlamydomonas reinhardtii.  Mol. Gen. Genet. 184, 460‑464.
2. Ford, C., S. Mitchell, and W.‑Y. Wang.  (1983).  Characterization of NADPH: Protochlorophyllide Oxidoreductase in the y‑7 and pc‑1 y‑7 mutants of Chlamydomonas reinhardtii.  Mol. Gen. Genet. 192, 290‑292
3. Ford, C., and M. M. Smith.  (1985).  Use of an oligonucleotide probe to detect transplacement of an amber mutation into a yeast histone H3 gene. Gene 37, 45‑52.
4. Sierks, M. R., C. Ford, P. J. Reilly, and B. Svensson. (1989). Site- directed mutagenesis at the active site Trp120 of Aspergillus awamori glucoamylase.  Protein Eng. 2, 621-625.
5. Sierks, M. R., C. Ford, P. J. Reilly, and B. Svensson. (1990). Catalytic mechanism of fungal glucoamylase as defeined by mutagenesis of Asp176, Glu179, and Glu180 in the enzyme from Aspergillus awamori.  Protein Eng. 3,: 193-198.
6. Zhao, J., C. Ford, C. Glatz, M. Rougvie, and S. Gendel. (1990). Polyelectrolyte precipitation of ß-galactosidase fusions containing polyaspartic acid tails. J. Biotechnol. 14, 273-284.
7. Evans, R., C. Ford, M. Sierks, Z. Nikolov, and B. Svensson. (1990). Deletion analysis of the O-glycosylated region of Aspergillus glucoamylase: effect on activity and thermal stability. Gene 91, 131-134.
8. Ford, C. F., I. Suominen, and C. E. Glatz.  (1991) Fusion tails for the recovery and purification of recombinant proteins.  Protein Expres. Purif. 2, 95-107.
9. Chen, L., C. Ford, and Z. Nikolov. (1991). Adsorption to starch of a ß-galactosidase fusion protein containing the starch-binding domain from Aspergillus glucoamylase. Gene 99, 121-126.
10. Sierks, M., C. Ford, P. J. Reilly, and B. Svensson. (1993)  Fuinctional roles and subsite locations of Leu 177, Trp 178, and Asn 182 of Aspergillus awamori glucoamylase determined by site-directed mutagenesis.  Protein Eng. 6, 75-79.
11. Suominen, I., C. Ford, D. Stachon, H. Heimo, M. Niederauer, H. Nurmeia, and C. Glatz. (1993) Enhanced recovery and purification of Aspergillus glucoamylase from Saccharomyces cerevcisiae by addition of poly(aspartic acid) tails.  Enzyme Microbiol. Technol. 15, 593-600.
12. Kusnadi, A. R., C. Ford, and Z. L. Nikolov. (1993) Production of a functional starch-binding domain of Aspergillus glucoamylase I in  Escherichia coli.  Gene 127, 193-197.
13. Bakir, U., P. M. Coutinho, P. A. Sullivan, C. Ford, and P. J. Reilly. (1993) Cassette mutagenesis of  Aspergillus awamori glucoamylase near its general acid residue to probe its catalytic and pH properties.  Protein Eng. 6, 939-946.
14. Chen, H.-M., U. Bakir, Ala mutant6P. J. Reilly, and C. Ford. (1994) Increased thermostability of Asn182 Aspergillus awamori glucoamylase.  Biotech. Bioeng. 43, 101-105.
15. Chen, H.-M., C. Ford, and P. J. Reilly.  (1994)  Substitution of asparagine residues in Aspergillus awamori glucoamylase by site-directed mutagenesis to eliminate N-glycosylateion and inactivation by deamidation.  Biochem. J. 301, 275-281.
16. Niederauer, M. Q., I. Suominen, M. A. Rougvie, C. F. Ford, and C. E. Glatz. (1994) Characterization and polyelectrolyte precipitation of ß-galactosidase containing genetic fusions of charged polypeptides.  Biotechnol. Prog. 10, 237-245.
17. Flory, N., M. Gorman, P. M. Coutinho, C. Ford, and P. J. Reilly. (1994) Thermosensitive mutants of Aspergillus awamori glucoamylase by random mutagenesis: inactivation kinetics and structural interpretation.  Protein Eng. 7, 1005-1012.
18. Libby, C. B., C. A. G. Cornett, P. J. Reilly, and C. Ford. (1994) Effect of amino acid deletions in the O-glycosylated region of Aspergillus awamori glucoamylase.  Protein Eng. 7, 1109-1114.
19. Chen, H.-M., C. Ford, and P. J. Reilly. (1995) Identification and elimination by site-directed mutagenesis of thermolabile aspartyl bonds in Aspergillus awamori glucoamylase.  Protein Eng. 8, 575-582.
20. Chen, L., P. Coutinho, Z. Nikolov, and C. Ford. (1995) Deletion analysis of the starch binding domain of Aspergillus glucoamylase.  Protein Eng. 8, 1049-1055.
21. Chen, H.-M., T. Panda, Y. Li, F. Bühler, C. Ford, and P. J. Reilly. (1996) Effect of replacing helical glycine residues with alanines on the thermostability and production of Aspergillus awamori glucoamylase.  Protein Eng. 9, 499-505.
22. Li, Y., P. J. Reilly, and C. Ford. (1997) Effect of introducing proline residues on the stability of  Aspergillus awamori glucoamylase.  Protein Eng.  10, 1199-1204.
23. Fang, T.-Y.,  P. M. Coutinho, P. J. Reilly, and C. Ford. (1998) Mutations to alter Aspergillus awamori selectivity. I. Y48F, Y116W, Y175F, R241K, S411A and S411G.  Protein Eng 11, 119-126.
24. Fang, T.-Y., P. M. Coutinho, P. J. Reilly, and C. Ford. (1998) Mutations to alter Aspergillus awamori glucoamylase selectivity II. Mutation of residues 119 and 121.  Protein Eng 11, 127-133.
25. Fang, Tsuei-Yun and Clark Ford. (1998) Protein engineering of Aspergillus awamori glucoamylase to increase its pH optimum.  Protein Eng. 11, 383-388.
26. Liu, Hsuan-Liang, Pedro M. Coutinho, Clark Ford, and Peter J. Reilly. (1998)  Mutations to alter Aspergillus awamori glucoamylase selectivity III. N20C/A27C, A27P, S30P, K108R, K108M, G137A, 311-314 loop, Y312W and S436P. Protein Eng. 11, 383-398.
27. Li, Yuxing, Pedro M. Coutinho, and Clark Ford. (1998) Effect on thermostability and catalytic activity of introducing disulfide bonds into Aspergillus awamori glucoamylase.  Protein Eng. 11, 661-667.
28. Allen, Martin J., Pedro M. Coutinho, and Clark F. Ford. (1998) Stabilization of Aspergillus awamori glucoamylase by proline substitution and combining stabilizaing mutations.  Protein Eng. 11, 783-788.
29. Allen, M., T.-Y. Fang, Y. Li, H.-L. Liu, H.-M. Chen, P. Coutinho, R. Honzatko, and C. Ford (Patent Issued: 2003) Protein Engineering of Glucoamylase to Increase pH Optimum, Substrate Specificity  and Thermostability.   U.S. Patent # 09/236,063.  Filed 1999.
30. Ford, C.F. (1999) Improving operating performance of glucoamylase by mutagenesis. Current Opinion in Biotechnology 10, 353-357.
31. Liu, Hsuan‑Liang, Yann Doleyres, Pedro M. Coutinho, Clark Ford, and Peter J. Reilly (2000)  Replacement and deletion mutations in the catalytic domain and belt region of Aspergillus awamori glucoamylase to enhance thermostability.   Protein Eng. 13, 655‑659.
32. Khan, Saber M., Peter.J. Reilly, and Clark Ford (2000) Thermal and Molecular Characterization of Aspergillus awamori Glucoamylase Catalytic and Starch‑Binding Domains. Starch/Stärke 52, 385‑397
33. Allen, Martin J., Erica B. Fuchs, and Clark F. Ford (2002). Asp238®Asn creates a novel consensus N-glycosylation site in Aspergillus awamori glucoamylase. Starch/Stärke 54, 385-392.
34. Cornett, Catherine A.G., Tsuei-Yun Fang, Peter J. Reilly, and Clark Ford (2003). Starch-binding domain shuffling in Aspergillus niger glucoamylase.  Protein Eng. 16, 521-529.
35. Wang, Yue, Erica Fuchs, Roberto Da Silva, Allison McDaniel, Janice Seibel, and Clark Ford. (2006).  Improvement of Aspergillus niger glucoamylase
36. McDaniel, Allison, Erica Fuchs, Ying Liu, and Clark Ford. (2008). Directed evolution of Aspergillus niger glucoamylase to increase thermostability.  Microbial Biotechnology 1, 523-531.
37. Warner, Christopher D., Julie A. Hoy, Taran C. Shilling, Michael J. Linnen, Nathaniel D. Ginder, Clark F. Ford, Richard B. Honzatko, and Peter J. Reilly (2010) Tertiary structure and characterization orf a glycoside hydrolase family 44 endoglucanase from Clostridium acetobutylicum.  Appl. Env. Micro. 76, 338-346.
38. Warner, Christopher D., Rica M. Go, Carolina Garcia-Salinas, Clark Ford, and Peter J. Reilly (2011) Kinetic characterization of a glycoside hydrolase family 44 xyloglucanase/endoglucanase from Ruminococcus flavefaciens FD-1. Enzyme Microb. Technol. 48, 27-32

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Last Updated: August, 2012.

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