Advances in Subculture
Application of the entomopathogenic fungi (EPF), as an alternative to conventional chemical pesticides, represents a safe sustainable crop protection method for integrated pest management (IPM) strategies. More than 700 species of EPF are used as biological control agents, with particular emphasis on Metarhizium spp. (Humber, 2008). Approximately 39% of mycoinsecticides based on genus Metarhizium as an active ingredient (Faria and Wriaght, 2007), probably due to their high host specificity, non-persistence, and eco-friendly with a unique mode of action against target pests. M. anisopliae showed high mortality rates against the stored grain pests Sitophilus granarius and S. oryza depending on conidial concentrations and the absence or presence of food (Ak, 2019), while evaluation of different strains of M. anisopliae against Spodoptera exigua and S. littoralis showed a high accumulative mortality rate (100%) against 2nd and 4th larval instars of S. littoralis and 1st and 3rd instars of S. exigua larvae (Anand and Tiwary, 2009; Han et al., 2014)
Sample Chapter(s)
Preface (483 KB)
Components of the Book:
  • Chapter 1
    Comparative study of fungal stability between Metarhizium strains after successive subculture
  • Chapter 2
    Motivations for use, identity and the vaper subculture a qualitative study of the experiences of Western Australian vapers
  • Chapter 3
    Risk of depression and self-harm in teenagers identifying with goth subculture a longitudinal cohort study
  • Chapter 4
    Simple propagation method for resident macrophages by co-culture and subculture, and their isolation from various organs
  • Chapter 5
    Enhanced pyruvate metabolism in plastids by overexpression of putative plastidial pyruvate transporter in Phaeodactylum tricornutum
  • Chapter 6
    Optimization of rhizogenesis for in vitro shoot culture of Pinus massoniana Lamb.
  • Chapter 7
    In vitro bioproduction and enhancement of moscatilin from a threatened tropical epiphytic orchid, Dendrobium ovatum (Willd.) Kraenzl
  • Chapter 8
    Cryopreservation of virus a novel biotechnology for long-term preservation of virus in shoot tips
  • Chapter 9
    Unravelling quality culture in higher education a realist review
  • Chapter 10
    Pillarization (‘Verzuiling’). On Organized ‘Self-Contained Worlds’ in the Modern World
Rana H. M. Hussien, Department of Botany, Faculty of Science, Zagazig University, Zagazig, Egypt

Jonine Jancey, Collaboration for Evidence, Research and Impact in Public Health, School of Public Health, Curtin University, Bentley, Western Australia 6102, Australia

Tama Leaver, School of Media, Creative Arts and Social Inquiry, Curtin University, Bentley, Western Australia, Australia

Katharina Wolf, School of Marketing, Curtin University, Bentley, Western Australia, Australia

Becky Freeman, School of Public Health, University of Sydney, Sydney, New South Wales, Australia.

and more...
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