TITLE:
In Vitro Propagation of Three Strawberry Varieties and Field Evaluation
AUTHORS:
Sussmita Karmaker, Md. Mukhtar Hossain, Md. Aminul Hoque, Md. Abdul Kaium, Tabaraka Binte Ali, Musfika Ferdous
KEYWORDS:
Strawberry, In Vitro, Propagation, Genotypes, Root Induction, Shoot Proliferation
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.14 No.11,
November
8,
2023
ABSTRACT: A study was done to produce a rapid in
vitro propagation of three strawberry genotypes and tested in the
field under Bangladeshi circumstances. Festival, RABI-3, and Neho strawberry
genotypes’ runner tips were cultivated in
vitro to induce root induction and multiple shoot proliferation. MS (Murashige and Skoog) media that were basally
containing three different concentrations at 1.0, 1.5, and 2.0 of BA
(6-benzyl adenine), KIN (6-furfuryl amino purine), or GA3 (gibberellic acid) at 0.5 mg/L increasing tips of the
runner was attained. The culture grew
on the medium provided with 1.5 mg/L 6-benzyl adenine and 0.5 mg/L 6-furfuryl amino acid to increase
shoot at the best level. Micro-cuttings were rooted on MS media at half
strength combined with 0.5 mg/L - 1.5 mg/L IBA (indole butyric acid) or IAA
(indole acetic acid). IBA attained 4 - 9 roots and 91% - 96% rooting at 1.0
mg/L. The resulting plantlets grew into hardy plants and took root in the
earth. The genotype festival had the highest response rate, followed by RABI-3
and Neho.