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has been cited by the following article:

• TITLE: Micro-environmentally Restricted Yeast Cell Growth within Ca-alginate Microbeads

  AUTHORS: Ivana Pajic-Lijakovic, Branko Bugarski, Branko Bugarski, Milenko Plavsic, Steva Levic, Ana Kalusevic, Viktor Nedovic

  KEYWORDS: Yeast Cells; Micro-environmental Restrictions; Ca-alginate Hydrogel Matrix; Structural Changes; Pattern of Volumetric Yeast Growth

  JOURNAL NAME: Engineering, Vol.4 No.10B, October 21, 2013

  ABSTRACT: Micro-environmental restriction effects to yeast cell growth obtained within Ca-alginate microbeads are considered. It is complex phenomenon influenced by: (1) relaxation of expanded polymer network around the cellular clusters, (2) forces generated by cell growth inside the beads and (3) interactions between solvent, network parts and cells. The resulting effects are measured experimentally by estimating volume of microbeads and yeast cell concentration as function of time of cultivation. Comparative analysis of dynamics of cell growth and increase of microbead volume through four regimes indicates that reversible and irreversible local structural changes of Ca-alginate hydrogel induces micro-environmental restrictions to cell growth. The mechanism of restrictions includes both mechanical and electrostatic effects.