TITLE:
Phytochemical Profiling with Antioxidant and Antimicrobial Screening of Amaranthus viridis L. Leaf and Seed Extracts
AUTHORS:
Saud Asif Ahmed, Sumaira Hanif, Tehreema Iftkhar
KEYWORDS:
Amaranthus viridis; Phytochemicals; DPPH. Lineolic Acid; Reducing Power; Leaf and Seed Extract
JOURNAL NAME:
Open Journal of Medical Microbiology,
Vol.3 No.3,
September
18,
2013
ABSTRACT: Methanol extracts of the dried leaves and seeds of Amaranthus viridis were collected and used for phytochemicals and antibacterial analysis. By detecting the MIC and zone inhibition, the antibacterial activity was determined against different bacterial and fungal strains. The extract yields from the leaves and seeds ranged 5.5-6.1 and 2.42%-3.72% w/w, respectively. Phytochemical investigation of this plant determines that tanins (6.07%-5.96%), saponins (53%-32%), alkaloids (13.14% - 11.42%), protiens (16.76%-24.51%) and glycosides (63.2%-32.3%) were rich in leaves. The extracts also contained appreciable levels of total phenolic contents (2.81-3.61 GAE, g/100 g), total flavanoid contents (18.4-5.42 QE, g/100 g) and DPPH free radical scavenging activity, showing IC50 (83.45-75.95 μg/mL) along with reducing power was calculated. The MIC of extracts ranged 178 - 645 μg/mL. The results of this study suggest the possibility of using the methanolic extracts in treating the diseases caused by the test organisms.