TITLE:
The diagnostic significance of glucose-6-phosphate isomerase (G6PI) antigen and anti-G6PI antibody in rheumatoid arthritis patients
AUTHORS:
Daren Yang, Huinan Ge, Jing Dong, Xiongxiong Zhu, Gang Sun, Weiguo Ouyang, Linhui Wang, Guoxing Zhang
KEYWORDS:
Glucose-6-Phosphate Isomerase (G6PI); G6PI Antibody; Rheumatoid Arthritis (RA)
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.8,
August
1,
2013
ABSTRACT:
Objective: To investigate whether glucose-6-phosphate isomerase
(G6PI) antigen and anti-G6PI antibodies could be applied for the clinical
diagnostic markers of rheumatoid arthritis (RA) and its associations with RA
activity states. Methods: The levels of G6PI antigens and anti-G6PI Abs in sera
from 176 RA patients in different states, 35 non-RA patients and 100 healthy
donors and in synovia fluids from 33 patients and 11 non-RA patients were
measured by ELISA. Results: The sensitivity and specificity of G6PI antigens in
the RA patients were 75.0% and 93.3%, respectively. The levels of serum G6PI
antigens in 176 RA patients were significantly higher than non-RA patients and
the health controls. Especially, there was a significant difference between the
active phase and the inactive phase in G6PI antigens levels. The levels of G6PI
antigens in synovia fluid were also significantly higher in RA groups than in
non-RA patients. With the values of the anti-G6PI Abs in sera, there were no
marked differences among RA, non-RA patients and health controls. Also, there
was no significant difference between the active phase and the inactive phase
in RA patients. However, there were no significant differences of G6PI and
anti-G6PI between RA patients and health controls in synovial fluid.
Conclusions: G6PI is highly correlated with the activity states of RA, and
could be applied for a clinical biomarker with high sensitivity and specificity
for the diagnosis of RA.