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Wang, H., Doll, J., Jiang, K., Cundiff, D., Czarnecki, J., Wilson, M., Ridge, K. and Soff, G. (2006) Differential binding of plasminogen, plasmin, and angiostatin 4.5 to cell surface beta-actin: Implications for cancer-mediated angiogenesis. Cancer Research, 66, 7211-7215.
doi:10.1158/0008-5472.CAN-05-4331
has been cited by the following article:
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TITLE:
Enzyme electrophoresis method in analysis of active components of haemostasis system
AUTHORS:
Ludmila Ostapchenko, Oleksiy Savchuk, Nataliia Burlova-Vasilieva
KEYWORDS:
Substrate-Containing Electrophoresis; Enzyme Electrophoresis; Haemostasis; Proteins Activity; Proteins Identification
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.2 No.1,
February
24,
2011
ABSTRACT: The novel modifications of substrate-containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis that can be used for the detection of proteases and its activators are reported. The protease/activator samples were separated on a protein substrate-SDS-polyacrylamide gel. To detect plasminogen activators fibrinogen and Glu-plasminogen were incorporated into the SDS-PAG followed by 1 h incubation at 37?C in thrombin solution (1 NIH/ml). After electrophoresis the gel was stained according to the standard protocol. To detect fibrin-unspecific plasminogen activators from snake venom incubation in thrombin solution was substituted for 12 h incubation in 50 mM Tris-HCl (pH 7.4). To detect fibrinogen-degrading enzymes fibrinogen-containing gel was used. Activity of protease/activator was visualized in the gel as clear bands against the dark background. These new techniques offer several advantages including determination of the quantity and activity of t-PA and urokinase, however cannot be recommended for precise quantification of activators; the total procedure is quite quick and simple; method is convenient tool for detection of novel protein-protein interactions in haemostasis system; the sensitivity of the method is ≤0.01 IU per track.
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