TITLE:
Protein Globule Formation in the Liver Graft during Cold Preservation for Liver Transplantation: Its Clinical and Pathological Significance
AUTHORS:
Changyin Feng, Tongmei He, Haijian Huang, Lijuan Qu, Yi Jiang, Wenhua Zhang, Zhiyong Zheng
KEYWORDS:
Hepatitis B Virus; Immunohistochemistry; Liver Transplantation; Liver Ischemia-Reperfusion Injury
JOURNAL NAME:
Open Journal of Pathology,
Vol.3 No.3,
June
28,
2013
ABSTRACT:
In liver
transplantation, liver graft ischemia-reperfusion injury occurs mainly due to
cold preservation and warm reperfusion. In this research, we study the
affection of plasma protein of the donor on liver graft during cold
preservation and warm reperfusion. In this study, 34 liver transplantations
were performed from 2007 to 2010, and the clinical data were collected
retrospectively from the Dongfang Hospital database. 34 specimens were
harvested from 34 liver grafts when graft trimming as Group A and 34 specimens
harvested from the same 34 liver grafts during liver transplantation surgery
but before abdominal closure as B group. All liver tissue specimens were fixed
with 40 g/L neutral formalin, embedded in paraffin. Light microscopy,
transmission electron microscopy, Periodic Acid-Schiff (PAS) stain and immunohistochemical
stain of IgG, IgM, IgA, C3d, C4d, Fib, C1q, and CD61 were used. In this study,
we found that eosinophilic bodies emerged in liver lobes during liver
transplantation which had not been reported previously in the literature. 1)
Protein globules were found exclusively in liver graft specimens. The globules
were round or oval with sharp edges, measured approximately 1.59 to 9.41 μm in
diameter, and were scattered in the liver sinusoids or space of Disse or hepatocyte
cytoplasm, were stained with IgG, IgM, IgA, Fib, C3d by immunohistochemical staining; 2) There was no statistical
significant difference of protein globules number between A group and B group
(P > 0.05); 3) IRI score of B group was not
correlated with protein globules number (P > 0.05). Protein globules contain plasma
composition, and may form during cold preservation.