TITLE:
Using of In-Situ Mercury Film Sensor Hyphenated with Affinity Voltammetry for High Throughput Drug-Protein Binding Studies
AUTHORS:
Ahmed K. Youssef, Deia Abd El-Hady
KEYWORDS:
Sensor; Affinity Voltammetry; Fenoprofen; Bovine Serum Albumin; Binding Constant
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.4 No.4,
April
29,
2013
ABSTRACT:
A new simple and reliable in-situ mercury film sensor coupled with
affinity differential pulse stripping voltammetry (ADSPV) or affinity cyclic
voltammetry (ACV) was investigated. The interaction of fenoprofen with bovine
serum albumin (BSA) onto the proposed electrochemical sensor was studied.
The nature of the electrochemical process of fenoprofen by cyclic
voltammetry was depicted. Reproducibility of the proposed method was checked
giving a precision of 0.073 standard deviation. The limit of detection and
limit of quantification were 7.0 and 22.0 nmol/L, respectively. Fenoprofen was
interacted with BSA by 1:1 stoichiometry to form electroinactive supramolecular
complex. The binding constant was precisely estimated by non-linear regression
analysis based on the shifting of analyte peak potentials. The proposed
experiments and data analysis could be used to investigate the drug-protein binding
constant within a short analysis time compared to other chromatographic
techniques.