TITLE:
The intrinsic or the extrinsic pathways of apoptosis in the epidermis after cladribine application?
AUTHORS:
Patrycja Chylinska-Wrzos, Ewelina Wawryk-Gawda, Marta Lis-Sochocka, Marian Jedrych, Miroslaw Lancut, Kamila Bulak, Joanna Leszcz-Stankiewicz, Barbara Jodlowska-Jedrych
KEYWORDS:
Cladribine; Apoptosis; Caspase 8; Caspase 9; Epidermis
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.6 No.3,
March
12,
2013
ABSTRACT:
Purpose: This study was aimed to
assess the expression of caspase 8 and caspase 9 in the epidermis, during
apoptosis after cladribine administration. Materials and Methods: The
experiment was carried on 10 Wistar rats. The animals were placed into 2 groups:
control group and experimental group. Animals in the control group, in addition
to standard feed and water, were injected with a physiological salt in a
quantity corresponding to the drug dosage, over the course of the experiment.
The experimental group animals were treated with cladribine in the dose of 0.07
mg/kg/24h, for 6 consecutive days in the morning, in 3 cycles with a 5-week gap
in administering the drug. After the completion of the experiment, the animals
were decapitated, and full thickness slices of skin were taken from all the
rats for immunohisto- chemical study. The results of the statistical surveys
were analysed in Statistica 10.0. The difference in intensity of expression of
caspase 9 and caspase 8 in the groups were investigated using the Chiquadrat
test. Statistical significance was considered at p