TITLE:
Biomonitoring of organic and inorganic chemicals in the hair of Vietnamese people via spectral and chromatographic analysis
AUTHORS:
Le Phuoc Cuong, Le Thi Xuan Thuy, Micheal Evgenev
KEYWORDS:
NAT; CYP 450; Pesticides; Chemical Elements; ICP-AES; SFE-GC/MS
JOURNAL NAME:
Journal of Biophysical Chemistry,
Vol.4 No.1,
February
28,
2013
ABSTRACT:
A
combination of spectral and chromatographic analytical methods has been executed
to monitor chemicals in the hair of Vietnamese people. Methods were developed for
the determination of some chemical elements in hair by inductively coupled-plasma
atomic emission spectroscopy with limits of detection reaching 1 - 100 μg·l－1.
A supercritical fluid extraction (SFE) and clean-up procedures were established
for isolating organochlorine pollutants (OCPs) and organophosphorus pesticides (OPPs)
from the hair of the Vietnamese test subjects. Eight OCPs and OPPs were determined
by SFE with carbon dioxide only and methanol-modified carbon dioxide extraction
at 350 bar and 45℃, followed by gas chromatography coupled to mass spectrometry.
The results indicated that OCPs can be extracted quantitatively using CO2 only, whereas OPPs require a modifier for extraction.
The limits of detection that were found were between 0.7 to 1.3 μg·g－1,
and good linearity (r2 > 0.9861) was achieved within the tested ranges. The activities of the N-acetyltransferase (NAT) and the monooxygenases cytochrome P450 (CYP 450) were determined and compared to the acetylation
and oxidation phenotypes of healthy Vietnamese
test subjects. The variations in the
concentration of chemical elements in human hair were shown to be dependent
upon the activities of the two-phase metabolic
system, on the individual’s gender and the duration of residence within
Russia.