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Morbt, N., Tomm, J., Feltens, R., Mogel, I., Kalkhof, S., Murugesan, K., Wirth, H., Vogt, C., Binder, H., Lehmann, I. and von Bergen, M. (2010) Chlorinated benzenes cause concomitantly oxidative stress and induction of apoptotic markers in lung epithelial cells (A549) at nonacute toxic concentrations. Journal of Proteome Research, 10, 363-378. doi:10.1021/pr1005718
has been cited by the following article:
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TITLE:
Biological implications of 2-chlorocyclohexa-2,5-diene-1,4-dione toward ribonuclease A
AUTHORS:
Albert R. Vaughn, Caitlin B. Redman, Sophia M. Kang, Jisook Kim
KEYWORDS:
Chlorobenzoquinone; PAH; Protein Modification; Ribonuclease A
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.1,
January
21,
2013
ABSTRACT: 2-Chlorocyclohexa-2,5-diene-1,4-dione
(CBQ) or 2-chloro1,4-benzquinone is one of the common metabolites of polycyclic
aromatic hydrocarbons generated through industrial processes. This report describes
the biological effects of CBQ toward ribonuclease A (RNase). We also investigated
the inhibition of RNase modifications and the reactivity of CBQ toward selected
amino acids. The study was carried out by incubating RNase or amino acids with
CBQ in a concentration- and a time-dependent manner at 37°C and pH 7.0. SDS-PAGE
results showed oligomerization as well as polymeric aggregation of RNase when
incubated with CBQ as early as in 10 min. CBQ-induced RNase modifications were
inhibited in the presence of NADH or ascorbic acid. CBQ reactivity toward selected
amino acids was also evaluated by determining the second-order rate constants for
the reactions of CBQ with selected amino acids. It was found that the reactivity
toward CBQ decreased in the order of lysine > threonine
> serine >> aspartate > cysteine.
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