S. H. Lee, D. H. Oh, W. S. Ahn, Y. Lee, J.-I. Choi and S. Y. Lee, “Production of Poly (3-Hydroxybutyrat-co-3-hydroxyhexanoate) by High-Cell-Density Cultiviation of Aeromonas hydrophila,” Biotechnology and Bioengineering, Vol. 67, No. 2, 2000, pp. 240-244. doi:10.1002/(SICI)1097-0290(20000120)67:2<240::AID-BIT14>3.0.CO;2-F

has been cited by the following article:

• TITLE: Polyhydroxyalkanoate Production by Pseudomonas putida KT217 on a Condensed Corn Solubles Based Medium Fed with Glycerol Water or Sunflower Soapstock

  AUTHORS: Jeremy Javers, Chinnadurai Karunanithy

  KEYWORDS: Polyhydroxyalkanoate; Pseudomonas putida; Condensed Corn Solubles; Glycerol; Soapstock

  JOURNAL NAME: Advances in Microbiology, Vol.2 No.3, September 24, 2012

  ABSTRACT: Pseudomonas putida KT217 was grown on a complex medium comprised of co-products of the ethanol and biodiesel industries to assess the organism's capability to produce medium-chain-length polyhydroxyalkanoate (mcl-PHA). The growth phase was carried out in a medium containing 400 g/L condensed corn solubles (CCS), supplemented with ammonium hydroxide as a nitrogen source. Following the exponential phase, co-products of the biodiesel industry (soapstock and glycerin) were fed into the reactor to trigger PHA production. When glycerin was added to the bioreactor (75 g/L total addition), the final cell dry weight (CDW) and PHA content were 30 g/L and 31%, respectively. The monomeric composition in the PHA formed was relatively uniform throughout incubation with 3-hydroxydecanoate dominating. When a total of 153 g/L of sunflower soapstock was added to the bioreactor in a fed-batch manner, the final CDW and PHA content were 17 g/L and 17%, respectively. Following addition of soapstock the monomeric composition of the polymer changed dramatically, with the 3-hydroxyoctanoate monomer becoming dominant and greater unsaturation present in the PHA.