Article citationsMore>>
A. Black, D. Francoeur, T. Rowe, J. Collins, D. Miller, T. Brown, M. David, S. Dunn, W. A. Fisher, N. Fleming, C. A. Fortin, E. Guilbert, L. Hanvey, A. Lalonde, R. Miller, M. Morris, T. O’Grady, H. Pymar, T. Smith and E. Henneberg, “Canadian Contraception Consensus,” Journal of Obstetrics and Gynaecology Canada, Vol. 26, No. 2, 2004, pp. 347-436.
has been cited by the following article:
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TITLE:
Development of Immunochemical Methods for Purification and Detection of the Steroid Drug Medroxyprogesterone Acetate
AUTHORS:
Alisa Bronshtein, Alex Krol, Haim Schlesinger, Miriam Altstein
KEYWORDS:
Medroxyprogesterone Acetate; ELISA; Immunoaffinity Chromatography; Sol-Gel; Pharmaceutical Residues; Residue Monitoring
JOURNAL NAME:
Journal of Environmental Protection,
Vol.3 No.7,
July
18,
2012
ABSTRACT: An immunochemical sol-gel-based immunoaffinity purification (IAP) method for purification and detection of the progestin drug medroxyprogesterone acetate (MPA) was developed. A polyclonal antibody (Ab) for MPA was generated, and two competitive (indirect and direct) sensitive enzyme-linked immunosorbent assays (ELISAs) for its detection were developed and implemented to determine the recovery and efficiency of the sol-gel based IAP method. The detection limits of the assays were 1.4 ± 0.2 ng·mL-1 (n = 4) and 4.0 ± 0.4 ng·mL-1 (n = 25) for the indirect and direct ELISAs, respectively. The Abs did not exhibit cross-reactivity with any other progestin or steroid hormone, with the exception of megestrol acetate, with which the Ab exhibited 76% cross-reactivity. The sol-gel IAP method successfully eliminated serum interference to a degree that enabled ELISA analysis of spiked serum samples. This method was also found fully compatible with subsequent chemical analytical methods, such as liquid chromatography followed by mass spectrometry (LC-MS/MS). The approaches developed in this study form a basis for analysis of MPA in biological samples and may be further used to study population exposure to MPA and to monitor MPA contamination in water samples.
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