TITLE:
Development and validation of HPTLC method for niacin and simvastatin in binary combination
AUTHORS:
Pravish Kumar Tiwari, Padmakar Sathe
KEYWORDS:
HPTLC; Methanol; Niacin; Simvastatin
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.1 No.2,
June
30,
2010
ABSTRACT: A simple, sensitive and validated HPTLC method has been developed to determine Niacin and simvastatin simultaneously in synthetic mixture form. Chromatographic separation was achieved on a RP18 plate using a mixture of Methanol: Water: Acetic acid (60:40:0.1) at a wavelength of 237 nm. Linearity of themethod was found to be in the concentration range of
5000.0-25000.0 μg/ml for niacin and 100.0-500.0 μg/ml for simvastatin with correlation coefficient greater than 0.999. The method can be used for simultaneous determination of Niacin and Simvastatin.