TITLE:
Release of Arabinose from Wheat Insoluble Arabinoxylan by the Action of α-L-Arabinofuranosidase in Synergism with Endo-Xylanases
AUTHORS:
Dominic W. S. Wong, Sarah Batt
KEYWORDS:
α-L-Arabinofuranosidase, Mono-Substituted Xylopyranosyl Residuet, Di-Substituted Xylopyranosyl Unit, Arabinoxylan Arabinofuranohydrolase (AXH)
JOURNAL NAME:
Advances in Enzyme Research,
Vol.11 No.4,
December
29,
2023
ABSTRACT: Wheat arabinoxylan (water-insoluble fraction) contains ~36% arabinose which may include both singly or doubly substitutions at C2/C3 of the Xylp units. α-L-Arabinofuranosidses (ABFs) of two GH families were analyzed for their respective activities on the hydrolysis of Xylp-Araf. BaABF (GH43) produced twice the yield of arabinose residues from the heteroxylan compared to AnABF (GH51) under the same reaction conditions. Two endo-xylanases (of GH10 and 11) also showed differential hydrolytic activities on the Xylp chain, with the GH10 XYN-ATM double the amount of reducing sugar yield (as xylose equivalent) than using the GH11 XYN-M3. When the ABF and XYN were combined in optimial ratios, a synergistic increase of 73.8% in arabinose yield was observed.