TITLE:
Micropropagation of Kaempferia angustifolia Roscoe via Direct Regeneration
AUTHORS:
Zuraida Ab Rahman, Ayu Nazreena Othman, Mohd Norfaizal Ghazalli, Nur Auni Syazalien Adlan
KEYWORDS:
BAP, NAA, Micropropagation, Kaempferia angustifolia, Direct Regeneration
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.13 No.6,
June
17,
2022
ABSTRACT: This study has established
an efficient and reproducible protocol to micropropagation Kaempferia angustifolia Roscoe via direct regeneration. The use of
young shoots as explants showed the best results compared to rhizome shoots,
where the young shoots showed a low percentage of contamination of 10% - 30%
(agar 6 g/L) and 45% - 55% (agar 3 g/L), respectively, compared to the use of
rhizome shoots, where the contamination rate exceeded 80%. For shoot
initiation, the combination of BAP (6 Benzylaminopurine) and NAA
(1-Naphthaleneacetic acid) showed higher results for the percentage of initial
shoots and number of micro shoots/explants compared to BAP with Kin (Kinetin).
The highest concentration of BAP (5 mg/L) combined with the lowest
concentration of NAA (0.5 mg/L) resulted in 90% of initial shoots and a number
of shoots/explants of 5.8. The highest number of shoots for micropropagation
was in treatment with 30 g/L sucrose that was segmented with 3 mg/L BAP + 0.5
mg/L NAA. For the number of roots, the highest
number of roots was 11.8 recorded at sucrose (45) with only BAP (1 mg/L) used
as the plant growth regulator, while the longest length of roots was 7 - 8 cm,
recorded both at sucrose with the combination of BAP and NAA.