TITLE:
Interaction Effect of Auxin and Cytokinin on in Vitro Shoot Regeneration and Rooting of Endangered Medicinal Plant Valeriana jatamansi Jones through Tissue Culture
AUTHORS:
Urooj Nazir, Zishan Gul, Ghulam Mujtaba Shah, Nayyar Iqbal Khan
KEYWORDS:
Valeriana jatamansi, Medicinal Plant, Tissue Culture, NAA, BAP
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.13 No.2,
February
22,
2022
ABSTRACT: Micropropagation of Valeriana jatamansi Jones by using small
segments of rhizome on full strength MS medium having various concentrations
and combinations of auxin Naphthaleneacetic acid (NAA) and cytokinin
Benzylaminopurine (BAP) was conducted. The highest mean shoot length (3.71 cm) was achieved when media was fortified with BAP 2 mg/L in combination
with NAA 1 mg/L. The highest mean leaf number i.e. 6.00 was observed when BAP was used alone at 2 mg/L. Average
root length (0.77 cm) was recorded when BAP 1.5 mg/L
along with NAA 0.5 mg/L was used. Maximum mean root numbers 2.57 were obtained
when BAP and NAA were used at equal concentrations i.e. 1.5 mg/L. Observed data
demonstrated that BAP up to 1 mg/L, 1.5 mg/L and 2 mg/L promotes shoot length,
leaf number and leaf growth when used along with NAA at 0 mg/L, 0.5 mg/L and 1 mg/L.
However lower quantities of both NAA (0 mg/L, 0.5 mg/L) and BAP (1 mg/L and 1.5
mg/L) produced significantly higher root length of Valeriana jatamansi Jones but the higher concentrations of plant
growth hormones BAP (2 mg/L, 3 mg/L) and NAA
(1 mg/L, 1.5 mg/L) were found unfavorable for increase in root length but the
root number increases at higher concentration of NAA (1 mg/L and 1.5 mg/L).