TITLE:
DNA Fidelity: Expression of a Monocot Promoter in a Dicot Plant
AUTHORS:
Luis María Suárez-Rodríguez, Hugh Mason, Joel Ramírez-Cabrera, Luis Jorge Saucedo, Miguel Angel Gómez-Lim, Charles Arntzen, Rodolfo López-Gómez
KEYWORDS:
Promoter, Fruit, Ripening, Tomato, Banana
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.13 No.1,
January
18,
2022
ABSTRACT: The knowledge generated from the identification of
plant promoters has been very important for plant biotechnology development.
The use of promoters in transgenic plants allows a reasonable level of
regulating protein expression. With the application of reporter genes, such as gusA (uidA,) the production of a colored protein, β-glucuronidase, can be detected and measured both qualitatively
and quantitatively, and the activity of the promoter can be assessed. In this
work we use a promoter of an abundant banana fruit protein gene Musa
acuminata Acidic Chitinase class III a monocot species, to drive expression
of gusA in a dicot species, like
tomato. We evaluated the monocot promoter capabilities by localizing and
quantifying β-glucuronidase (GUS)
expression through fluorometric assays during tomato fruit ripening. Our
results suggest that this promoter could be used for specifically strong fruit
protein expression in dicot plants.