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has been cited by the following article:

• TITLE: Effect of Excipients on Recombinant Interleukin-2 Stability in Aqueous Buffers

  AUTHORS: A. S. Prakasha Gowda, Andrew D. Schaefer, Terry K. Schuck

  KEYWORDS: Interleukin-2, Excipients, Stability, RP-HPLC, SDS-PAGE

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.12 No.10, October 29, 2021

  ABSTRACT: In order to retain structural and functional integrity, protein medicines are frequently stabilized with excipients in aqueous solutions. The goal of this investigation was to see how stable IL-2 is with excipients that are acceptable for cell therapy. We investigated the time-dependent stability of commercially available recombinant IL-2 in aqueous solutions (CTS, RPMI, PBS, and water) at different temperatures [2°C - 8°C, room temperature (20°C ± 2°C) and 37°C] in the presence of excipients (EDTA, methionine, histidine, and glycine) over a period of up to 30 days. To detect and quantify IL-2, reversed phase high performance liquid chromatography was employed. Electrophoresis on a sodium dodecyl sulfate polyacrylamide gel was used to assess conformational stability. We discovered that IL-2 stability was improved in aqueous solutions including excipients, and that it may have retained its biological activity and sterility in these conditions.