TITLE:
Correlation of Quantitative Changes in Bone Marrow Lymphocyte Subpopulations with Some Rhabdomyosarcoma Prognosis Factors in Children
AUTHORS:
Olga P. Kolbatskaya, Tatiana V. Gorbunova, Svetlana V. Chulkova, Anna A. Khachatryan, Nikolai N. Tupitsyn, Vladimir G. Polyakov
KEYWORDS:
Rhabdomyosarcoma, Bone Marrow, Children, Lymphocyte Subpopulations
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.11 No.5,
October
29,
2021
ABSTRACT: Rationale: The known prognosis factors for rhabdomyosarcoma
(RMS) in children do not always explain the unsatisfactory
outcome of treatment. Changes in the subpopulation composition of Bone
Marrow (BM) effector cells during the
development of RMS may indicate new directions for the search for prognostic factors and points for the impact of
targeted therapy. Purpose: To identify correlations between quantitative changes
in the levels of subpopulations of T, B and NK-lymphocytes of BM and known risk
factors for RMS in children. Objects: The study included 31
patients. The main group included 16 patients with RMS, average age—6.8 ± 1.0 years,
while children 1 - 10 years old—13 (81.3%), over 10 years old—3 (18.8%) people,
girls and boys were 8 people each. The
embryonic variant of RMS was established in 10 (62.5%) cases, the
alveolar variant—in 4 (25%) cases, in two patients (12.5%), the histological variant was not established.
In 12 (75%) patients, an unfavorable localization
of the RMS (parameningeal, extremities, prostate, bladder) was revealed,
in 4 patients (25%), the localization of the tumor was regarded as favorable. Patients with T2b—13
(81.2%) and T2a—2 (12.5%) stages prevailed. Regional and distant metastases
were detected in 10 (52.6%) patients. The comparison group included 15 children
in whom the presence of malignant neoplasia was excluded, the average age was
8.4 ± 1.5 years, 11 boys (73.7%) and 4 girls (26.3%). Methods: All patients underwent morphological (myelogram counting) and
immunological (quantitative analysis of lymphocytic subpopulations) bone
marrow studies. Immunophenotyping in all patients was carried out by direct immunofluorescence
using a triple fluorescent label. Results: Significant differences in the levels of
subpopulations of BM T-lymphocytes were found when comparing the values of the
main group, distributed by localization and histological variant, with the data
obtained in the control group of patients. For example, the percentage of CD3+ T cells with the co-stimulatory molecule CD28+
was significantly higher in patients with parameningeal RMS (p = 0.010). Conclusion: Each clinical group of patients
has its own individual immunological characteristics. The results obtained by
us can be considered indicative and regarded as starting points for further
study of the peculiarities of the subpopulation composition of BM in patients
with RMS.