TITLE:
Genetic Variation among Seedling of Pumpkins Genotypes through SDS-Page
AUTHORS:
Muhammad Ikram, Niaz Ali, Muhammad Ishaq, Muhammad Romman, Yasir Islam, Naeem Khan
KEYWORDS:
Genetic Diversity, SDS-PAGE, Pumpkin, Cluster Analysis
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.12 No.5,
May
12,
2021
ABSTRACT: Genetic diversity in 30 genotypes of Pumpkins was collected from unexplored
mountainous areas of Khyber Pakhtunkhwa, Pakistan was investigated through
biochemical characterization. For biochemical characterization, Sodium Dodecyl
Sulphate Polyacrylamide Gel Electrophoresis was carried out. The seed proteins
were resolved on 7.5% and 15% polyacrylamide gel. A total of 35% genetic
disagreement was observed in the collected lines with linkage distances ranging
from 0.00 - 0.83 (percent disagreement). Similarly, cluster analysis sorted total
germplasm on the basis of 12 bands (total bands) into eight clusters. Present
study revealed a considerable amount of genetic diversity explored in pumpkin
germplasm, Cluster analysis exhibited moderate level of genetic diversity; to
broaden the gene pool. Further collection of the important germplasm is needed to be used in
the development of improved cultivars with respect to quality and quantity.