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has been cited by the following article:

• TITLE: Genetic Variation among Seedling of Pumpkins Genotypes through SDS-Page

  AUTHORS: Muhammad Ikram, Niaz Ali, Muhammad Ishaq, Muhammad Romman, Yasir Islam, Naeem Khan

  KEYWORDS: Genetic Diversity, SDS-PAGE, Pumpkin, Cluster Analysis

  JOURNAL NAME: American Journal of Plant Sciences, Vol.12 No.5, May 12, 2021

  ABSTRACT: Genetic diversity in 30 genotypes of Pumpkins was collected from unexplored mountainous areas of Khyber Pakhtunkhwa, Pakistan was investigated through biochemical characterization. For biochemical characterization, Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis was carried out. The seed proteins were resolved on 7.5% and 15% polyacrylamide gel. A total of 35% genetic disagreement was observed in the collected lines with linkage distances ranging from 0.00 - 0.83 (percent disagreement). Similarly, cluster analysis sorted total germplasm on the basis of 12 bands (total bands) into eight clusters. Present study revealed a considerable amount of genetic diversity explored in pumpkin germplasm, Cluster analysis exhibited moderate level of genetic diversity; to broaden the gene pool. Further collection of the important germplasm is needed to be used in the development of improved cultivars with respect to quality and quantity.