TITLE:
Regeneration and Genetic Fidelity Analysis of Chlorophytum borivilianum Using Flower Stalk as Explant Source
AUTHORS:
Nishant Kaushal, Anshu Alok, Monika Kajal, Kashmir Singh
KEYWORDS:
Chlorophytum borivilianum, In Vitro Regeneration, SCoT, ISSR, Genetic Fidelity
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.12 No.4,
April
16,
2021
ABSTRACT: Chlorophytum borivilianum is a critically endangered plant well known for its
medicinal properties for diabetes mellitus, diarrhea, arthritis, sterility, and
erectile dysfunction, etc. Due to low viability and long dormancy of seeds, in vitro regeneration is
required for large scale cultivation of this plant. In the present study,
direct plant regeneration was optimized using flower stalk as explant. Nodal
segments of flower stalk were sterilized and kept for direct regeneration on
different combinations of BAP and KIN supplemented media. The highest,
15.27 ± 1.14 number of shoots were produced on medium containing BAP (2 mg/L)
per nodal segment. The multiple shoot clumps regenerated from flower stalk were
separated carefully and kept on rooting media. A maximum of 16.87 ± 1.53 roots
per plant was observed in MS media having 0.5 mg/L of NAA. The
rooted plantlets were shifted into the pot containing soilrite for hardening
and acclimatization. The genetic stability of hardened plants was confirmed by
start codon targeted, and inter simple sequence repeats molecular markers. All
the 18 randomly selected plantlets showed similar genetic homogeneity to the
mother plant. It is the first report on in vitro regeneration along with the
genetic fidelity analysis of the regenerated plantlets from flower Stalk of C. borivilianum. As the
standardized method of regeneration and mass multiplication is quite efficient
and genetically stable, the protocol will be useful for the large-scale
production of C. borivilianum to meet the market demand.