Eisenberg, D., Luthy, R. and Bowie, J.U. (1997) VERIFY3D: Assessment of Protein Models with Three-Dimensional Profiles. Methods in Enzymology, 277, 396-404.
https://doi.org/10.1016/S0076-6879(97)77022-8

has been cited by the following article:

• TITLE: Structure Prediction and Enzymatic Properties of Phytase phyS

  AUTHORS: Yueming Li, Hongqing Xu, Jianchun Xu, Ruilian Pang, Bingzheng Xu

  KEYWORDS: Structural Prediction, Enzymatic Properties, Phytase

  JOURNAL NAME: Advances in Enzyme Research, Vol.7 No.4, December 24, 2019

  ABSTRACT: Phytase is a kind of enzyme that hydrolyzes phytic acid and its salts to produce inositol and phosphoric acid. As a new feed additive, phytase has great potential in animal nutrition and environmental protection. Because of its good stability, large-scale production and high activity, microbial phytase has become a hot spot in industrial application. Here, we reported the predicted structure and enzymatic properties of a phytase from Bacillus subtilis, which was named as phyS. It was clear that the optimal temperature is 35°C, and the optimal pH is 8. Meanwhile, the enzyme activity was kept at above 90% in the range of pH 8 - 9, this result demonstrated that phyS is an alkaline phytase. This study lays a foundation for the extensive application of phyS.