TITLE:
The Extract Obtained from Two Radish Cultivars (Raphanus sativus L. and Raphanus sativus L. var. radikula) Using Liquid Nitrogen Is Germination of Sterile Oats (Avena sterilis Subsp. sterilis) and Allelopathic Effect on Seedling Growth
AUTHORS:
Duygu Sisek, Suleyman Turkseven, Suleyman Topal
KEYWORDS:
Allelopathy, Avena sterilis, Antep Radish, Liquid Nitrogen
JOURNAL NAME:
Open Access Library Journal,
Vol.6 No.7,
July
30,
2019
ABSTRACT: The purpose of this study is to determine the allelopathic effects of extracts obtained by using liquid nitrogen from the plant of Antep radish (Raphanus sa-tivus L.) and little radish (Raphanus sativus L. var. radikula) on germination and seedling growth of sterile oats. The experiments were carried out in six groups with root, stem and root stem mixed extracts of radish plants (Antep and little radish). A novel method that we developed based on the principle of liquid nitrogen crushing was used for extraction. According to this method, Antep radish and little radish plant parts were frozen with liquid nitrogen aid and then crushed to powder. These powders obtained were stored at ？20？C for nine months until the experiments were established. The aqueous solutions prepared at specific concentrations (0%, 1%, 2%, 4%, 8%, 16%) from the ob-tained radish powders were applied as 5 ml to per each petri dishes contain 10 seeds on Whatman No: 1 double layer filter paper. Experiments were carried out in six replicates for each concentration. The prepared petri dishes were kept in the incubator at 15？C for 15 days. At the end of the experimental period, the number of germination of the seeds of sterile wild oat plant was evaluated by measuring the length of seedling root and stem. As a result, as the concentra-tion of allelopathic solutions increased in all experimental groups, the number of germination and root and stem lengths of sterile wild oats decreased. Alt-hough allelopathic effects in all three experimental groups were observed in terms of germination number and seedling lengths, the most significant effects were observed in the results of Antep radish root experiments. In this experi-ment group, the inhibition rate of root and stem length of weed seeds at a con-centration of 16% were 86% and 82%, respectively, when compared to the control. In addition, the germination number was reduced by 74.36% in the seeds at the highest concentration compared to the control. As a result of the study, it was determined that samples of radish plant crushed with liquid nitro-gen were stored for a long time and showed high allelopathic effect on sterile wild oats plant.