TITLE:
Genetic Diversity of Tomato (Solanum lycopersicum L.) Begomovirus in Togo
AUTHORS:
Assion Sétu Mivedor, Justin Simon Pita, Kossikouma Djodji Adjata, Jérôme Duclercq, Yawovi Mawuena Dieudonné Gumedzoe
KEYWORDS:
Begomoviruses, Direct Sequencing, Phylogenetic Relationships, Tomato, Wild Plants
JOURNAL NAME:
Agricultural Sciences,
Vol.8 No.12,
December
29,
2017
ABSTRACT:
Geminiviruses, in particular the members of the genus Begomovirus , are considered
to be a major phytosanitary problem for tomato crops production in
the world. They are responsible for yield losses of up to 20% to 100%. Regrettably,
Togo is not spared from this situation. This work aims to show the genetic
diversity of the begomoviruses affecting tomato crops production in
Togo and their relationship with other begomoviruses. To achieve these objectives,
307 samples of tomato leaves and wild plant species with typical virus
symptoms were collected in the Maritime, Plateaus, Central, Kara and Savannah
regions and submitted to PCR analysis. The results revealed the presence
of begomovirus in 25.40% of the analyzed samples. The PCR products obtained
were submitted to direct sequencing. Phylogenetic analysis of sequences
of DNA-A different regions of begomovirus identified in this work
with that of other begomoviruses showed a nucleotide identity of 96% respectively
for Tomato leaf curl Togo virus-Fontem, Tomato Leaf Curl Togo Virus ,
Ageratum leaf curl Cameroon Alphasatellite; 98% respectively to Tomato leaf
curl Nigeria virus , Ageratum leaf curl Cameroon virus , Tomato leaf curl Cameroon
virus-Fontem, Ageratum leaf curl Cameroon virus and 99% respectively
to Tomato leaf curl Kumasi virus , Pepper yellow vein Mali virus Bazegahot
and Pepper yellow vein Mali virus-Ouaga. These results suggest a high
degree of genetic diversity of tomato begomoviruses identified in Togo.