Stein, D.C., Silver, L.E., Clark, V.L. and Young, F.E. (1983) Construction and Characterization of a New Shuttle Vector, pLES2, Capable of Functioning in Escherichia coli and Neisseria gonorrhoeae. Gene, 25, 241-247.

has been cited by the following article:

• TITLE: Genomic Recombination Enhances Pathogenic Factors in the Periodontopathogenic Bacterium Eikenella corrodens

  AUTHORS: Fariha Jasin Mansur, Kazunori Yamada, Natsumi Morishige, Hiroyuki Azakami

  KEYWORDS: Eikenella corrodens, Genomic Recombination, Biofilm, Hemolysis, Hemagglutination

  JOURNAL NAME: Advances in Microbiology, Vol.7 No.4, April 14, 2017

  ABSTRACT: We reported previously that plasmid-mediated genomic recombination at the pilin gene locus increased hemagglutination activity, growth rate, biofilm formation, hemolytic activity, and adherence to epithelial cells in Eikenella corrodens 23834. To determine whether these enhancements were common in this bacterium, we introduced the recombinase gene ORF4 into seven clinically isolated strains. Genomic recombination at the type IV pilin gene locus was observed in strains 1080, L9B6, L8Ao3, and RV2 (group A), but not in strains 261-2, 612-L, and 257-4 (group B). Similarly, group A strains displayed changed colony morphology following loss of type IV pili, which was not observed in group B. Group A strains showed also enhanced hemagglutination activity, growth rate, hemolytic, activity and biofilm formation. These results suggest that ORF4-induced genomic recombination at the pilin gene locus is a general phenomenon in a part of E. corrodens, which likely stimulates patho-genicity and virulence.