TITLE:
Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (Carthamus tinctorius L.) cv. NARI-H-15
AUTHORS:
Jeyachandran Vijayakumar, Ponnirul Ponmanickam, P. Samuel, D. N. P. Sudarmani, J. Pandiarajan
KEYWORDS:
Carthamus Tinctorius L., Meta-Topolin, N-(2-chloro-4-pyridyl-N’-phenylurea), Micropropagation, Organogenesis
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.4,
March
16,
2017
ABSTRACT: The effect of meta-Topolin (mT) was assessed to
develop a reliable protocol for efficient
plant regeneration of safflower (Carthamus tinctorius L.) cv. NARI-H-15.
For micropropagation, 7 - 9 days old shoot-tip explants cultured on MS basal
medium supplemented with 3.0 mg/L meta-Topolin (mT) + 0.5 mg/L CPPU showed
97.7% adventitious shoot formation (42.4 shootlets) than node after 45 days of
culture. For organogenesis, the seedling explants of immature leaf cultured on 1.5 mg/L CPPU or 1.5 mg/L NAA
fortified medium produced high amount of callus than cotyledon and stem
calli after 60 days of culture. However, MS
basal medium fortified with 4.0 mg/L mT + 1.5 mg/L CPPU was found beneficial to stimulate 100%
organogenic response (74.7 shootlets) from immature leaf calli than
cotyledon and stem derived calli after 45 days of culture. The healthy
plantlets obtained from micropropagation and organogenesis process cultured on
1/4 MS basal salts, 1.5% sucrose (w/v) and 0.8%
agar (w/v) medium supplemented with NAA (1.5 mg/L) and mT (0.1 mg/L)
produced maximum of 96% (12.8 rootlets) and 84% (7.3 rootlets) adventitious
rooting, respectively than mT and CPPU tested medium. However, maximum of 67%
and 42% survival rate was noticed when in
vitro raised plants from micropropagation and organogenesis were hardened
in pots containing soil mix and maintained under green house condition. This
optimized regeneration protocol might be helpful in regeneration of new genotypes
and cultivars of safflower to improve agronomic traits through in vitro selection process and Agrobacterium-mediated genetic transformation
system.