TITLE:
Biobenefication of Oxide Minerals from Bacillus subtilis Using FTIR and MALDI-TOF Techniques
AUTHORS:
H. Sarvamangala, Vinay B. Raghavendra, S. T. Girisha
KEYWORDS:
Biobeneficiation, FTIR, MALDI-TOF, Bacillus subtilis, Oxide Minerals
JOURNAL NAME:
Journal of Environmental Protection,
Vol.8 No.2,
February
28,
2017
ABSTRACT: Biobeneficiation processes
involving the separation of valuable minerals from ores and materials using
conventional flotation and flocculation methods have been shown to be promising
in recent years. There is an enormous potential to utilize microorganisms as
flocculating agents, flotation collectors and/or depressants. The study
involves Biobeneficiation of oxide minerals using Bacillus subtilis.
Characterization of minerals (hematite, corundum, calcite and quartz) was
carried out through XRD, EDAX and FTIR techniques. FTIR of minerals before and
after interaction with cells, cell free extract and extracellular proteins was
carried out and it has been found that there is a shift or change in the peaks
of functional groups. In presence of protein adsorption, amide peaks were found
and in case of polysaccharide adsorption, carboxyl peaks were found which justify
the flotation and flocculation results. MALDI-TOF was carried out to confirm
the molecular weights of the extracted proteins and it was found that molecular
weight of proteins on interaction with minerals was higher than that of
uninteracted minerals.