Lee, J.Y., Yoo, C., Jun, S.Y., Ahn, C.Y. and Oh, H.M. (2010) Comparison of Several Methods for Effective Lipid Extraction from Microalgae. Bioresource Technology, 10, 75-77.
http://dx.doi.org/10.1016/j.biortech.2009.03.058

has been cited by the following article:

• TITLE: Evaluation of Various Extraction Techniques for Efficient Lipid Recovery from Thermo-Resistant Microalgae, Hindakia, Scenedesmus and Micractinium Species—Comparison of Lipid Extraction Methods from Microalgae

  AUTHORS: Melih Onay, Cagla Sonmez, Huseyin A. Oktem, Meral Yucel

  KEYWORDS: Lipid Extraction, Thermo-Resistant Green Microalgae, Cell Disruption Techniques, Ultrasonication, Biodiesel

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.7 No.2, January 29, 2016

  ABSTRACT: In recent years, photosynthetic microalgae regained attention for biodiesel production. For efficient utilization of microalgae, a number of criteria including a strain with high biomass and lipid productivities and employment of effective and reliable methods for oil extraction from the obtained biomass should be met. Recently, we have isolated and identified three thermo-resistant green microalgae strains, namely; Scenedesmus sp. ME02, Hindakia tetrachotoma ME03 and Mic-ractinium sp. ME05. In this study, we compared percent lipid content of thermos-tolerant mic-roalgal strains using the following solvent extraction methods: Soxhlet, Bligh and Dyer and Folch methods with or without assisted cell disruption techniques including lyophilization, homogenization, ultrasonication, bead and microwave-assisted. The highest increase in lipid yield was obtained with a combination of lyophilization and ultrasonication techniques together with Soxhlet method: 27% of total dry weight for Micractinium sp. ME05. We conclude that lyophilization and ultrasonication are effective assistance methods for lipid extraction from thermo-resistant microalgae.