TITLE:
Evaluation of Various Extraction Techniques for Efficient Lipid Recovery from Thermo-Resistant Microalgae, Hindakia, Scenedesmus and Micractinium Species—Comparison of Lipid Extraction Methods from Microalgae
AUTHORS:
Melih Onay, Cagla Sonmez, Huseyin A. Oktem, Meral Yucel
KEYWORDS:
Lipid Extraction, Thermo-Resistant Green Microalgae, Cell Disruption Techniques, Ultrasonication, Biodiesel
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.7 No.2,
January
29,
2016
ABSTRACT: In recent years, photosynthetic microalgae
regained attention for biodiesel production. For efficient utilization of
microalgae, a number of criteria including a strain with high biomass and lipid
productivities and employment of effective and reliable methods for oil
extraction from the obtained biomass should be met. Recently, we have isolated
and identified three thermo-resistant green microalgae strains, namely; Scenedesmus sp. ME02, Hindakia tetrachotoma ME03 and Mic-ractinium
sp. ME05. In this study, we compared
percent lipid content of thermos-tolerant mic-roalgal
strains using the following solvent extraction methods: Soxhlet, Bligh and Dyer
and Folch methods with or without assisted cell disruption techniques including
lyophilization, homogenization, ultrasonication, bead and microwave-assisted.
The highest increase in lipid yield was obtained with a combination of
lyophilization and ultrasonication techniques together with Soxhlet method: 27%
of total dry weight for Micractinium sp. ME05. We conclude that
lyophilization and ultrasonication are effective assistance methods for lipid
extraction from thermo-resistant microalgae.