TITLE:
Bacterial Groups Concerned with Maturing Process in Manure Production Analyzed by a Method Based on Restriction Fragment Length Polymorphism Analysis
AUTHORS:
Katsuji Watanabe, Naoto Horinishi, Kunimasa Matumoto, Akihiro Tanaka, Kenichi Yakushido
KEYWORDS:
Maturing Phase, Manure Production, Microchip Electrophoresis, Multiple Enzyme Restriction Fragment Length Polymorphism Analysis, The Most Probable Number Method
JOURNAL NAME:
Advances in Microbiology,
Vol.5 No.13,
December
10,
2015
ABSTRACT: Composting is a biological aerobic decomposition process consisted from
different phases. Although the Japanese Standards for manure recommended that
it took at least 6 months to complete the maturing phase, there was no reliable
ground. In order to find out shortening method of the maturing phase, the
microorganisms concerned with a progress of the maturing was determined by using
the most probable number method (MPN) and PCR-RFLP of the 16S rDNA, which was
found effective to provide numbers and taxonomy of polymyxin B resistant
bacterial groups in the former paper [1]. Compared to the numbers after thermophilic phase, those of
Actinobacteria, δ-proteobacteria, and
the other gram negative bacteria increased to 50 times, 20 times, and 105 times, respectively, after maturing phase, while those of Bacillus spp., and α and β-proteobacteria decreased to 1/10, and
1/105 after maturing phase. Numbers of the other Fumicutes, and γ-proteobacteria remained in the same
revel. Actinobacteria, δ-proteobacteria,
and the other gram negative bacteria might be concerned with a progress of the
maturing phase, because these bacterial groups were detected and enumerated due
to their proliferation ability. Although number of Acitinobacteria might be
underestimated because of a PCR bias, the method was found effective for the
purpose to monitor bacteria actively proliferated in culture medium.