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http://dx.doi.org/10.3791/50360

has been cited by the following article:

• TITLE: Colonization of Rice Roots by a Green Fluorescent Protein-Tagged Isolate of Ustilaginoidea virens

  AUTHORS: Mebeaselassie Andargie, Luoye Li, Aiqing Feng, Jianxiong Li

  KEYWORDS: Endophytes, Plant-Fungal Interactions, Rhizosphere, Root Colonization, Ustilaginoidea virens

  JOURNAL NAME: American Journal of Plant Sciences, Vol.6 No.14, September 10, 2015

  ABSTRACT: The fungus U. virens is the causal agent of rice false smut disease. The green fluorescent protein (GFP) was used to mark this fungus in order to visualize and analyze the colonization and infection processes in vivo. Using epifluorescence microscopy colonization and infection on rice roots were visualized in vivo. After inoculation for 2 to 15 d, it was observed that the conidia and their germ-tubes had penetrated into epidermis of young roots. The hyphae were found inside the root xylem 18 d after inoculation. Generally, the transformed fungus colonized the rhizosphere, the cortex as well as the vascular tissues with symptoms of root necrosis observed. The results of this work show that U. virens colonize not only rice panicles but also the roots.