Daniel, P.T., Pun, K.T., Ritschel, S., Sturm, I., Holler, J., D?rken, B., et al. (1999) Expression of the Death Gene Bik/Nbk Promotes Sensitivity to Drug-Induced Apoptosis in Corticosteroid-Resistant T-Cell Lymphoma and Prevents Tumor Growth in Severe Combined Immunodeficient Mice. Blood, 94, 1100-1107.

has been cited by the following article:

• TITLE: Angiotensin-(1-7) Changes Apoptosis-Related Genes Expression in Human Breast Cancer Cell Line T47D

  AUTHORS: Cheryl Alecrim Santos, Gabriela Soares da Silva Brito, Silvana Aparecida Alves Corrêa de Noronha, Samuel Marcos Ribeiro de Noronha, Suma Imura Shimuta, Clovis Ryiuchi Nakaie, Ismael Dale Cotrim Guerreiro da Silva

  KEYWORDS: Angiotensin-(1-7), Breast Cancer Cells, Apoptosis, qPCR Array

  JOURNAL NAME: Journal of Cancer Therapy, Vol.5 No.14, December 17, 2014

  ABSTRACT: Angiotensin-(1-7) [Ang-(1-7)] is a heptapeptide of the renin-angiotensin system with vasodilator and anti-proliferative properties. In the present study, we aim to investigate whether Ang-(1-7) induces apoptosis in breast cancer cells and whether the altered expression of apoptosis-related genes is involved in this process. Human breast cell line T47D was treated with angiotensin-(1-7) and angiotensin II (Ang II). Cell proliferation and apoptosis were quantified using hemocytometer and flow cytometry, respectively. The expression of 84 apoptosis-related genes was evaluated through qPCR array. Ang-(1-7), as opposed to Ang II, decreased proliferation and increased apoptosis in T47D cells. Moreover, many pro-apoptotic genes were up-regulated, such as BAK1, BAX, BCL2L1, BID and BIK. In addition, some anti-apoptotic genes as AKT1 and XIAP were down-regulated by heptapeptide. Although a deeper study should be performed, our results support the hypothesis that Ang-(1-7) could change the expression of several genes related to apoptosis, interfering directly in the molecular pathways associated with the survival of breast cancer cells.