TITLE:
Angiotensin-(1-7) Changes Apoptosis-Related Genes Expression in Human Breast Cancer Cell Line T47D
AUTHORS:
Cheryl Alecrim Santos, Gabriela Soares da Silva Brito, Silvana Aparecida Alves Corrêa de Noronha, Samuel Marcos Ribeiro de Noronha, Suma Imura Shimuta, Clovis Ryiuchi Nakaie, Ismael Dale Cotrim Guerreiro da Silva
KEYWORDS:
Angiotensin-(1-7), Breast Cancer Cells, Apoptosis, qPCR Array
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.5 No.14,
December
17,
2014
ABSTRACT: Angiotensin-(1-7) [Ang-(1-7)] is a
heptapeptide of the renin-angiotensin system with vasodilator and
anti-proliferative properties. In the present study, we aim to investigate
whether Ang-(1-7) induces apoptosis in breast cancer cells and whether the altered
expression of apoptosis-related genes is
involved in this process. Human breast cell line T47D was treated with
angiotensin-(1-7) and angiotensin II (Ang II). Cell proliferation and
apoptosis were quantified using hemocytometer and flow cytometry, respectively.
The expression of 84 apoptosis-related genes was evaluated through qPCR array.
Ang-(1-7), as opposed to Ang II, decreased proliferation and increased apoptosis
in T47D cells. Moreover, many pro-apoptotic genes were up-regulated, such as BAK1, BAX, BCL2L1, BID and BIK. In addition, some anti-apoptotic genes as AKT1 and XIAP were down-regulated
by heptapeptide. Although a deeper study should be performed, our results
support the hypothesis that Ang-(1-7) could change the expression of several
genes related to apoptosis, interfering directly in the molecular pathways
associated with the survival of breast cancer cells.