TITLE:
Probing the Cell Cycle with Flow Cytometry
AUTHORS:
George D. Wilson
KEYWORDS:
Flow Cytometry, Cell Cycle, DNA Profile, Bromodeoxyuridine, Cyclins
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.7 No.9,
July
21,
2014
ABSTRACT: Flow cytometry is a versatile technique to study different aspects of the cell cycle from subpopulations of cells to detailed cell kinetic information. In this paper a basic review of cell kinetic parameters is presented followed by detailed descriptions of the different flow cytometric methodologies that can be used to extract pertinent information for a particular study. The methodologies range from simple DNA profile analysis, the use of bromodeoxyuridine to cell cycle-associated proteins such as the cyclins.