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has been cited by the following article:

• TITLE: Leptin Causes the Early Inhibition of Glycolysis- and TCA Cycle-Related Genes in the Brain of Ob/Ob Mice to Restore Fertility

  AUTHORS: Carlos Fernandes Baptista, Samuel Marcos Ribeiro de Noronha, Maria de Nazareth Gamboa Ritto, Eduardo Henrique da Silva Freitas, Melquíades Pereira Júnior, Mauro Abi Haidar, Ismael Dale Cotrim Guerreiro da Silva, Silvana Aparecida Alves Corrêa de Noronha, Marisa Teresinha Patriarca

  KEYWORDS: PCOS, Obesity, Leptin, Glycolysis, TCA Cycle, Gene Expression

  JOURNAL NAME: American Journal of Molecular Biology, Vol.4 No.3, July 16, 2014

  ABSTRACT: Introduction: Polycystic ovarian syndrome (PCOS) is undoubtedly the commonest androgen disorder in woman’s fertile period and certainly one of the most prevalent causes of anovulation. The syndrome has an estimated prevalence of 4% - 10% among women of childbearing age. Previously, our group demonstrated the effect of gonadal white adipose tissue transplantation from wild-type lean and fertile female mice to isogenic obese anovulatory ob/ob mice. These complex metabolic interrelationships between obesity and PCOS have yet to be fully understood. The aim of this study was to evaluate the effect of gonadal white adipose tissue (WAT) transplantation from the wild-type lean and fertile female mice to isogenic obese, anovulatory mice (Lep ob/Lep ob) on the expression of glycolysis- and TCA cycle-related genes and obtain a general view of the glucose metabolism in the brain of these animals. Methods: Fifteen ob/ob mice ranging from 2 to 3 months of age were divided into 3 experimental groups: control normal weight (n = 5), obese control (n = 5) and obese 7 days leptin treated (n = 5). The whole brains of the mice were processed for RNA extraction. The samples from each group were used to perform PCR assays using an array plate containing 84 primers to study the glucose metabolism-related genes. Results: The glycolysis- and TCA cycle-related genes were significantly downregulated. The most significantly affected genes were as follows: for glycolysis (fold regulation with p