Article citationsMore>>
Freeman Jr., T.C., Black, J.L., Bray, H.G., Dagliyan, O., Wu, Y.I., Tripathy, A., Dokholyan, N.V., Leisner, T.M. and Parise, L.V. (2013) Identification of novel integrin binding partners for calcium and integrin binding protein 1 (CIB1): Structural and thermodynamic basis of CIB1 promiscuity. Biochemistry, 52, 7082-7090.
http://dx.doi.org/10.1021/bi400678y
has been cited by the following article:
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TITLE:
Interaction of calcium- and integrin-binding protein 1 with integrin α11 and its possible involvement in pulmonary fibrosis
AUTHORS:
Koji Yoshida, Ah-Mee Park, Shingen Ozaki, Hiroshi Munakata
KEYWORDS:
Calcium- and Integrin-Binding Protein 1; Fibrosis; Integrin α11; Yeast Two-Hybrid
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.4 No.1,
February
27,
2014
ABSTRACT:
Integrin α11
(ITGA11) is one of the collagen-binding integrin α chains; however, its biological significance remains unknown. To determine
the functions of ITGA11, we performed a yeast two-hybrid screen using the
cytoplasmic domain of ITGA11 as bait and transformed an EGY48 yeast strain with
the bait-containing plasmid using the plasmid from a human lung fibroblast
cDNA library. This screen identified calcium- and integrin-binding protein 1 (CIB1)
as prey. Recombinant ITGA11 and CIB1 were expressed in mammalian cells and
used in coimmunoprecipitation experiments, which showed that full-length
ITGA11 and CIB1 are also associated in
vivo. Over-expression of CIB1 in the human lung myofibroblast MRC-5 cells
decreased the expression of α-smooth
muscle actin and fibronectin. Using a mouse model of pulmonary fibrosis (bleomycin-treatment),
we detected elevated expression of CIB1 in lung tissues compared with
controls. These data suggest that CIB1 may regulate pulmonary fibrosis in
concert with IT-GA11.
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