TITLE:
A study on the transmission of helicobacter pylori from food prepared and consumed under hygienic and unhygienic conditions: A first study using biopsy samples
AUTHORS:
Khawaja Shakeel Ahmed, Basheer Madompoyil, Janak Dulari Ahi, Aleem Ahmed Khan, Santosh Kumar Tiwari, Mohammed Aejaz Habeeb
KEYWORDS:
Helicobacter pylori; Hygiene; PCR; 16SrRNA; Food; Biopsy
JOURNAL NAME:
Health,
Vol.6 No.4,
February
20,
2014
ABSTRACT:
Objective: A comparative analysis on transmission of Helicobacter pylori in people who consumed
food prepared in both hygienic and unhygienic conditions. Method: The study was carried out in
1000 people across India. The Ratio of Male: female was 700: 300 with age group
ranging from 20 - 39. Three gastric biopsies were collected for identification
of Helicobacter pylori. PCR
(Polymerase Chain Reaction) amplification was done by using 16srRNA primers. Result: The analysis
showed the prevalence of H. pylori as
70.8% in individuals frequently consuming food prepared under less hygienic condition
(street vendor’s food) whereas 60% (less prevalence) in individuals consuming
food prepared under hygienic conditions. Conclusion: The results showed that most of the food that we consume
daily is not involved much in the transmission of Helicobacter pylori. The study also showed that the street vendor’s
food is not directly involved in the transmission of this bacterium. The
unhygienic preparation of food and poor personal hygiene play a vital role in
the transmission of these bacteria.